600-401-416

MYL12A antibody from Rockland Immunochemicals, Inc.
MLCB, MRCL3, MRLC3, MYL2B
Western blot
Data presented on provider website
ELISA
Recommended by provider
Immunoprecipitation
Recommended by provider
Immunohistochemistry
Supportive data in Antibodypedia

Antibody data

Product number
600-401-416
Provider
Rockland Immunochemicals, Inc.
Proper citation
Rockland Cat#600-401-416, RRID:AB_217940
Product name
Anti-Myosin pS19/pS20 (RABBIT) Antibody - 600-401-416
Provider product page
Rockland Immunochemicals, Inc. - 600-401-416
Antibody type
Polyclonal
Description
This affinity purified antibody is directed against the regulatory light chain of smooth and non-muscle myosin. The antibody is phosphospecific and detects monophosphorylated and diphosphorylated forms of the protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  Antiserum was first purified against the phosphorylated form of the immunizing peptide.  The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.  This phosphospecific polyclonal antibody is specific for the phosphorylated pS19/pS20 form of the protein, depending on the source origin of the protein.  Reactivity with non-phosphorylated myosin light chain is less than 1% by ELISA.  Cross reactivity is expected with myosin light chain from human and mouse.  Reactivity with the protein from other species has not been determined.  However, the sequence of the immunogen is nearly identical in mammalian and avian species.  BLAST search analysis was used to determine that the smooth and non-muscle forms of myosin regulatory light chain have identical sequences.  Cross reactivity is expected.
Reactivity
Human
Host
Rabbit
Conjugate
Unconjugated
Isotype
IgG
Vial size
100 µl
Concentration
1.0 mg/ml
Storage
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Handling
Dry Ice
Provider Type Product Number
- No reagents -