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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [45]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- AF566 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Neuropilin-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse and rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs less than 1% cross-reactivity with recombinant rat Neuropilin-2 is observed.
- Reactivity
- Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Gli3 Regulates Vomeronasal Neurogenesis, Olfactory Ensheathing Cell Formation, and GnRH-1 Neuronal Migration.
PlexinA1 is crucial for the midline crossing of callosal axons during corpus callosum development in BALB/cAJ mice.
Foxc2 is essential for podocyte function.
Molecular determinants of nephron vascular specialization in the kidney.
Axonal Growth of Midbrain Dopamine Neurons is Modulated by the Cell Adhesion Molecule ALCAM Through Trans-Heterophilic Interactions with L1cam, Chl1, and Semaphorins.
Neuropilin-1 expression in adipose tissue macrophages protects against obesity and metabolic syndrome.
Suppression of Retinal Neovascularization by Inhibition of Galectin-1 in a Murine Model of Oxygen-Induced Retinopathy.
Neuropilin 1 Involvement in Choroidal and Retinal Neovascularisation.
Neuropilin-1 promotes Hedgehog signaling through a novel cytoplasmic motif.
Silencing of galectin-1 inhibits retinal neovascularization and ameliorates retinal hypoxia in a murine model of oxygen-induced ischemic retinopathy.
Nrp2 is sufficient to instruct circuit formation of mitral-cells to mediate odour-induced attractive social responses.
Rewiring the taste system.
Neuropilin-1 and the Positions of Glomeruli in the Mouse Olfactory Bulb.
NOVA2-mediated RNA regulation is required for axonal pathfinding during development.
Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization.
Neural crest-derived SEMA3C activates endothelial NRP1 for cardiac outflow tract septation.
VEGF189 binds NRP1 and is sufficient for VEGF/NRP1-dependent neuronal patterning in the developing brain.
Inhibition of neuronal apoptosis and axonal regression ameliorates sympathetic atrophy and hemodynamic alterations in portal hypertensive rats.
Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing.
Astrocyte-encoded positional cues maintain sensorimotor circuit integrity.
Brain endothelial cells control fertility through ovarian-steroid-dependent release of semaphorin 3A.
Heparin binding VEGF isoforms attenuate hyperoxic embryonic lung growth retardation via a FLK1-neuropilin-1-PKC dependent pathway.
Imatinib inhibits VEGF-independent angiogenesis by targeting neuropilin 1-dependent ABL1 activation in endothelial cells.
Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas.
Neuropilin 1 (NRP1) hypomorphism combined with defective VEGF-A binding reveals novel roles for NRP1 in developmental and pathological angiogenesis.
Fluorochrome-based definition of naturally occurring Foxp3(+) regulatory T cells of intra- and extrathymic origin.
T cells generated in the absence of a thoracic thymus fail to establish homeostasis.
Id2 and Id3 maintain the regulatory T cell pool to suppress inflammatory disease.
Stability and function of regulatory T cells is maintained by a neuropilin-1-semaphorin-4a axis.
Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions.
The embryonic mouse hindbrain as a qualitative and quantitative model for studying the molecular and cellular mechanisms of angiogenesis.
The embryonic mouse hindbrain as a qualitative and quantitative model for studying the molecular and cellular mechanisms of angiogenesis.
Semaphorin 3A suppresses tumor growth and metastasis in mice melanoma model.
Neuropilin-1 is expressed by breast cancer stem-like cells and is linked to NF-κB activation and tumor sphere formation.
Neuropilin 1 is expressed on thymus-derived natural regulatory T cells, but not mucosa-generated induced Foxp3+ T reg cells.
Neuropilin 1 deficiency on CD4+Foxp3+ regulatory T cells impairs mouse melanoma growth.
IL-17-producing invariant NKT cells in lymphoid organs are recent thymic emigrants identified by neuropilin-1 expression.
Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development.
Alphav beta3 integrin limits the contribution of neuropilin-1 to vascular endothelial growth factor-induced angiogenesis.
BIG-2 mediates olfactory axon convergence to target glomeruli.
VEGF promotes vascular sympathetic innervation.
Bone marrow cells recruited through the neuropilin-1 receptor promote arterial formation at the sites of adult neoangiogenesis in mice.
Modulating Sema3A signal with a L1 mimetic peptide is not sufficient to promote motor recovery and axon regeneration after spinal cord injury.
Semaphorin and neuropilin co-expression in motoneurons sets axon sensitivity to environmental semaphorin sources during motor axon pathfinding.
Gating of Sema3E/PlexinD1 signaling by neuropilin-1 switches axonal repulsion to attraction during brain development.
Taroc EZM, Naik AS, Lin JM, Peterson NB, Keefe DL Jr, Genis E, Fuchs G, Balasubramanian R, Forni PE
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Jan 8;40(2):311-326
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Jan 8;40(2):311-326
PlexinA1 is crucial for the midline crossing of callosal axons during corpus callosum development in BALB/cAJ mice.
Hossain MM, Tsuzuki T, Sakakibara K, Imaizumi F, Ikegaya A, Inagaki M, Takahashi I, Ito T, Takamatsu H, Kumanogoh A, Negishi T, Yukawa K
PloS one 2019;14(8):e0221440
PloS one 2019;14(8):e0221440
Foxc2 is essential for podocyte function.
Nilsson D, Heglind M, Arani Z, Enerbäck S
Physiological reports 2019 May;7(9):e14083
Physiological reports 2019 May;7(9):e14083
Molecular determinants of nephron vascular specialization in the kidney.
Barry DM, McMillan EA, Kunar B, Lis R, Zhang T, Lu T, Daniel E, Yokoyama M, Gomez-Salinero JM, Sureshbabu A, Cleaver O, Di Lorenzo A, Choi ME, Xiang J, Redmond D, Rabbany SY, Muthukumar T, Rafii S
Nature communications 2019 Dec 13;10(1):5705
Nature communications 2019 Dec 13;10(1):5705
Axonal Growth of Midbrain Dopamine Neurons is Modulated by the Cell Adhesion Molecule ALCAM Through Trans-Heterophilic Interactions with L1cam, Chl1, and Semaphorins.
Bye CR, Rytova V, Alsanie WF, Parish CL, Thompson LH
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Aug 21;39(34):6656-6667
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Aug 21;39(34):6656-6667
Neuropilin-1 expression in adipose tissue macrophages protects against obesity and metabolic syndrome.
Wilson AM, Shao Z, Grenier V, Mawambo G, Daudelin JF, Dejda A, Pilon F, Popovic N, Boulet S, Parinot C, Oubaha M, Labrecque N, de Guire V, Laplante M, Lettre G, Sennlaub F, Joyal JS, Meunier M, Sapieha P
Science immunology 2018 Mar 16;3(21)
Science immunology 2018 Mar 16;3(21)
Suppression of Retinal Neovascularization by Inhibition of Galectin-1 in a Murine Model of Oxygen-Induced Retinopathy.
Yang N, Zhang W, He T, Xing Y
Journal of ophthalmology 2017;2017:5053035
Journal of ophthalmology 2017;2017:5053035
Neuropilin 1 Involvement in Choroidal and Retinal Neovascularisation.
Fernández-Robredo P, Selvam S, Powner MB, Sim DA, Fruttiger M
PloS one 2017;12(1):e0169865
PloS one 2017;12(1):e0169865
Neuropilin-1 promotes Hedgehog signaling through a novel cytoplasmic motif.
Pinskey JM, Franks NE, McMellen AN, Giger RJ, Allen BL
The Journal of biological chemistry 2017 Sep 15;292(37):15192-15204
The Journal of biological chemistry 2017 Sep 15;292(37):15192-15204
Silencing of galectin-1 inhibits retinal neovascularization and ameliorates retinal hypoxia in a murine model of oxygen-induced ischemic retinopathy.
Yang N, Zhang W, He T, Xing Y
Experimental eye research 2017 Jun;159:1-15
Experimental eye research 2017 Jun;159:1-15
Nrp2 is sufficient to instruct circuit formation of mitral-cells to mediate odour-induced attractive social responses.
Inokuchi K, Imamura F, Takeuchi H, Kim R, Okuno H, Nishizumi H, Bito H, Kikusui T, Sakano H
Nature communications 2017 Jul 21;8:15977
Nature communications 2017 Jul 21;8:15977
Rewiring the taste system.
Lee H, Macpherson LJ, Parada CA, Zuker CS, Ryba NJP
Nature 2017 Aug 17;548(7667):330-333
Nature 2017 Aug 17;548(7667):330-333
Neuropilin-1 and the Positions of Glomeruli in the Mouse Olfactory Bulb.
Zapiec B, Bressel OC, Khan M, Walz A, Mombaerts P
eNeuro 2016 Sep-Oct;3(5)
eNeuro 2016 Sep-Oct;3(5)
NOVA2-mediated RNA regulation is required for axonal pathfinding during development.
Saito Y, Miranda-Rottmann S, Ruggiu M, Park CY, Fak JJ, Zhong R, Duncan JS, Fabella BA, Junge HJ, Chen Z, Araya R, Fritzsch B, Hudspeth AJ, Darnell RB
eLife 2016 May 25;5
eLife 2016 May 25;5
Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization.
Rama N, Dubrac A, Mathivet T, Ní Chárthaigh RA, Genet G, Cristofaro B, Pibouin-Fragner L, Ma L, Eichmann A, Chédotal A
Nature medicine 2015 May;21(5):483-91
Nature medicine 2015 May;21(5):483-91
Neural crest-derived SEMA3C activates endothelial NRP1 for cardiac outflow tract septation.
Plein A, Calmont A, Fantin A, Denti L, Anderson NA, Scambler PJ, Ruhrberg C
The Journal of clinical investigation 2015 Jul 1;125(7):2661-76
The Journal of clinical investigation 2015 Jul 1;125(7):2661-76
VEGF189 binds NRP1 and is sufficient for VEGF/NRP1-dependent neuronal patterning in the developing brain.
Tillo M, Erskine L, Cariboni A, Fantin A, Joyce A, Denti L, Ruhrberg C
Development (Cambridge, England) 2015 Jan 15;142(2):314-9
Development (Cambridge, England) 2015 Jan 15;142(2):314-9
Inhibition of neuronal apoptosis and axonal regression ameliorates sympathetic atrophy and hemodynamic alterations in portal hypertensive rats.
Ezkurdia N, Raurell I, Rodríguez S, González A, Esteban R, Genescà J, Martell M
PloS one 2014;9(1):e84374
PloS one 2014;9(1):e84374
Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing.
Lee M, Kiefel H, LaJevic MD, Macauley MS, Kawashima H, O'Hara E, Pan J, Paulson JC, Butcher EC
Nature immunology 2014 Oct;15(10):982-95
Nature immunology 2014 Oct;15(10):982-95
Astrocyte-encoded positional cues maintain sensorimotor circuit integrity.
Molofsky AV, Kelley KW, Tsai HH, Redmond SA, Chang SM, Madireddy L, Chan JR, Baranzini SE, Ullian EM, Rowitch DH
Nature 2014 May 8;509(7499):189-94
Nature 2014 May 8;509(7499):189-94
Brain endothelial cells control fertility through ovarian-steroid-dependent release of semaphorin 3A.
Giacobini P, Parkash J, Campagne C, Messina A, Casoni F, Vanacker C, Langlet F, Hobo B, Cagnoni G, Gallet S, Hanchate NK, Mazur D, Taniguchi M, Mazzone M, Verhaagen J, Ciofi P, Bouret SG, Tamagnone L, Prevot V
PLoS biology 2014 Mar;12(3):e1001808
PLoS biology 2014 Mar;12(3):e1001808
Heparin binding VEGF isoforms attenuate hyperoxic embryonic lung growth retardation via a FLK1-neuropilin-1-PKC dependent pathway.
Esquibies AE, Karihaloo A, Quaggin SE, Bazzy-Asaad A, Cantley LG
Respiratory research 2014 Mar 19;15:32
Respiratory research 2014 Mar 19;15:32
Imatinib inhibits VEGF-independent angiogenesis by targeting neuropilin 1-dependent ABL1 activation in endothelial cells.
Raimondi C, Fantin A, Lampropoulou A, Denti L, Chikh A, Ruhrberg C
The Journal of experimental medicine 2014 Jun 2;211(6):1167-83
The Journal of experimental medicine 2014 Jun 2;211(6):1167-83
Neuropilin 1 expression correlates with differentiation status of epidermal cells and cutaneous squamous cell carcinomas.
Shahrabi-Farahani S, Wang L, Zwaans BM, Santana JM, Shimizu A, Takashima S, Kreuter M, Coultas L, D'Amore PA, Arbeit JM, Akslen LA, Bielenberg DR
Laboratory investigation; a journal of technical methods and pathology 2014 Jul;94(7):752-65
Laboratory investigation; a journal of technical methods and pathology 2014 Jul;94(7):752-65
Neuropilin 1 (NRP1) hypomorphism combined with defective VEGF-A binding reveals novel roles for NRP1 in developmental and pathological angiogenesis.
Fantin A, Herzog B, Mahmoud M, Yamaji M, Plein A, Denti L, Ruhrberg C, Zachary I
Development (Cambridge, England) 2014 Feb;141(3):556-62
Development (Cambridge, England) 2014 Feb;141(3):556-62
Fluorochrome-based definition of naturally occurring Foxp3(+) regulatory T cells of intra- and extrathymic origin.
Petzold C, Steinbronn N, Gereke M, Strasser RH, Sparwasser T, Bruder D, Geffers R, Schallenberg S, Kretschmer K
European journal of immunology 2014 Dec;44(12):3632-45
European journal of immunology 2014 Dec;44(12):3632-45
T cells generated in the absence of a thoracic thymus fail to establish homeostasis.
Smolarchuk C, Zhu LF, Chan WF, Anderson CC
European journal of immunology 2014 Aug;44(8):2263-73
European journal of immunology 2014 Aug;44(8):2263-73
Id2 and Id3 maintain the regulatory T cell pool to suppress inflammatory disease.
Miyazaki M, Miyazaki K, Chen S, Itoi M, Miller M, Lu LF, Varki N, Chang AN, Broide DH, Murre C
Nature immunology 2014 Aug;15(8):767-76
Nature immunology 2014 Aug;15(8):767-76
Stability and function of regulatory T cells is maintained by a neuropilin-1-semaphorin-4a axis.
Delgoffe GM, Woo SR, Turnis ME, Gravano DM, Guy C, Overacre AE, Bettini ML, Vogel P, Finkelstein D, Bonnevier J, Workman CJ, Vignali DA
Nature 2013 Sep 12;501(7466):252-6
Nature 2013 Sep 12;501(7466):252-6
Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions.
van Gils JM, Ramkhelawon B, Fernandes L, Stewart MC, Guo L, Seibert T, Menezes GB, Cara DC, Chow C, Kinane TB, Fisher EA, Balcells M, Alvarez-Leite J, Lacy-Hulbert A, Moore KJ
Arteriosclerosis, thrombosis, and vascular biology 2013 May;33(5):911-9
Arteriosclerosis, thrombosis, and vascular biology 2013 May;33(5):911-9
The embryonic mouse hindbrain as a qualitative and quantitative model for studying the molecular and cellular mechanisms of angiogenesis.
Fantin A, Vieira JM, Plein A, Maden CH, Ruhrberg C
Nature protocols 2013 Feb;8(2):418-29
Nature protocols 2013 Feb;8(2):418-29
The embryonic mouse hindbrain as a qualitative and quantitative model for studying the molecular and cellular mechanisms of angiogenesis.
Fantin A, Vieira JM, Plein A, Maden CH, Ruhrberg C
Nature protocols 2013 Feb;8(2):418-29
Nature protocols 2013 Feb;8(2):418-29
Semaphorin 3A suppresses tumor growth and metastasis in mice melanoma model.
Chakraborty G, Kumar S, Mishra R, Patil TV, Kundu GC
PloS one 2012;7(3):e33633
PloS one 2012;7(3):e33633
Neuropilin-1 is expressed by breast cancer stem-like cells and is linked to NF-κB activation and tumor sphere formation.
Glinka Y, Mohammed N, Subramaniam V, Jothy S, Prud'homme GJ
Biochemical and biophysical research communications 2012 Sep 7;425(4):775-80
Biochemical and biophysical research communications 2012 Sep 7;425(4):775-80
Neuropilin 1 is expressed on thymus-derived natural regulatory T cells, but not mucosa-generated induced Foxp3+ T reg cells.
Weiss JM, Bilate AM, Gobert M, Ding Y, Curotto de Lafaille MA, Parkhurst CN, Xiong H, Dolpady J, Frey AB, Ruocco MG, Yang Y, Floess S, Huehn J, Oh S, Li MO, Niec RE, Rudensky AY, Dustin ML, Littman DR, Lafaille JJ
The Journal of experimental medicine 2012 Sep 24;209(10):1723-42, S1
The Journal of experimental medicine 2012 Sep 24;209(10):1723-42, S1
Neuropilin 1 deficiency on CD4+Foxp3+ regulatory T cells impairs mouse melanoma growth.
Hansen W, Hutzler M, Abel S, Alter C, Stockmann C, Kliche S, Albert J, Sparwasser T, Sakaguchi S, Westendorf AM, Schadendorf D, Buer J, Helfrich I
The Journal of experimental medicine 2012 Oct 22;209(11):2001-16
The Journal of experimental medicine 2012 Oct 22;209(11):2001-16
IL-17-producing invariant NKT cells in lymphoid organs are recent thymic emigrants identified by neuropilin-1 expression.
Milpied P, Massot B, Renand A, Diem S, Herbelin A, Leite-de-Moraes M, Rubio MT, Hermine O
Blood 2011 Sep 15;118(11):2993-3002
Blood 2011 Sep 15;118(11):2993-3002
Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development.
Lin FJ, Chen X, Qin J, Hong YK, Tsai MJ, Tsai SY
The Journal of clinical investigation 2010 May;120(5):1694-707
The Journal of clinical investigation 2010 May;120(5):1694-707
Alphav beta3 integrin limits the contribution of neuropilin-1 to vascular endothelial growth factor-induced angiogenesis.
Robinson SD, Reynolds LE, Kostourou V, Reynolds AR, da Silva RG, Tavora B, Baker M, Marshall JF, Hodivala-Dilke KM
The Journal of biological chemistry 2009 Dec 4;284(49):33966-81
The Journal of biological chemistry 2009 Dec 4;284(49):33966-81
BIG-2 mediates olfactory axon convergence to target glomeruli.
Kaneko-Goto T, Yoshihara S, Miyazaki H, Yoshihara Y
Neuron 2008 Mar 27;57(6):834-46
Neuron 2008 Mar 27;57(6):834-46
VEGF promotes vascular sympathetic innervation.
Marko SB, Damon DH
American journal of physiology. Heart and circulatory physiology 2008 Jun;294(6):H2646-52
American journal of physiology. Heart and circulatory physiology 2008 Jun;294(6):H2646-52
Bone marrow cells recruited through the neuropilin-1 receptor promote arterial formation at the sites of adult neoangiogenesis in mice.
Zacchigna S, Pattarini L, Zentilin L, Moimas S, Carrer A, Sinigaglia M, Arsic N, Tafuro S, Sinagra G, Giacca M
The Journal of clinical investigation 2008 Jun;118(6):2062-75
The Journal of clinical investigation 2008 Jun;118(6):2062-75
Modulating Sema3A signal with a L1 mimetic peptide is not sufficient to promote motor recovery and axon regeneration after spinal cord injury.
Mire E, Thomasset N, Jakeman LB, Rougon G
Molecular and cellular neurosciences 2008 Feb;37(2):222-35
Molecular and cellular neurosciences 2008 Feb;37(2):222-35
Semaphorin and neuropilin co-expression in motoneurons sets axon sensitivity to environmental semaphorin sources during motor axon pathfinding.
Moret F, Renaudot C, Bozon M, Castellani V
Development (Cambridge, England) 2007 Dec;134(24):4491-501
Development (Cambridge, England) 2007 Dec;134(24):4491-501
Gating of Sema3E/PlexinD1 signaling by neuropilin-1 switches axonal repulsion to attraction during brain development.
Chauvet S, Cohen S, Yoshida Y, Fekrane L, Livet J, Gayet O, Segu L, Buhot MC, Jessell TM, Henderson CE, Mann F
Neuron 2007 Dec 6;56(5):807-22
Neuron 2007 Dec 6;56(5):807-22
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Recombinant Human, Mouse, and Rat Neuropilin-1 by Western Blot. Western blot shows 25 ng of Recombinant Mouse Neuropilin-1 (Catalog # 5994-N1), Recombinant Rat Neuropilin-1 Fc Chimera (Catalog # 566-N1) and Recombinant Human Neuropilin-1 (Catalog # 3870-N1). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse/Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Neuropilin-1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Neuropilin-1 in Embryonic Rat Spinal Cord. Neuropilin-1 was detected in immersion fixed frozen sections of embryonic rat spinal cord (15 d.p.c.) using 5 µg/mL Goat Anti-Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Neuropilin-1 in Rat Spinal Cord. Neuropilin-1 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). Specific staining was localized to the dorsal horn. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Neuropilin-1 in Rat Brain. Neuropilin-1 was detected in immersion fixed paraffin-embedded sections of rat brain (hippocampus) using Goat Anti-Mouse/Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal cell bodies and projections. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Neuropilin-1 in Rat Brain. Neuropilin-1 was detected in immersion fixed paraffin-embedded sections of rat brain (thalamus) using Goat Anti-Mouse/Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal cell bodies and projections. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Neuropilin-1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Rat Neuropilin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
