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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- 700281 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NIK Recombinant Rabbit Monoclonal Antibody (3H31L48)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody is predicted to react with Rhesus monkey, mouse, rat, bovine, equine and chicken based on sequence homology.
- Antibody clone number
- 3H31L48
- Concentration
- 0.5 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NIK in FLAG-tagged NIK in transfected 293 lysates (lane 2) and anti-FLAG used as a control (lane 1) using a NIK recombinant rabbit monoclonal antibody (Product # 700281) at a dilution of 15 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NIK in NIK-transfected 293 cells (right) and untransfected cells (left) using a NIK recombinant rabbit monoclonal antibody (Product # 700281) at a dilution of 5 µg/mL followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000 (green) and nuclei stained using Hoescht (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NIK in NIK-transfected 293 cells (right) and untransfected cells (left) using a NIK recombinant rabbit monoclonal antibody (Product # 700281) at a dilution of 5 µg/mL followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000 (green) and nuclei stained using Hoescht (blue).
