Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- sc-13162 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-13162, RRID:AB_628089
- Product name
- Anti-CDC20
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references ATP is required for the release of the anaphase-promoting complex/cyclosome from inhibition by the mitotic checkpoint.
Identification of novel human Cdt1-binding proteins by a proteomics approach: proteolytic regulation by APC/CCdh1.
Inhibitory factors associated with anaphase-promoting complex/cylosome in mitotic checkpoint.
Roles of the anaphase-promoting complex/cyclosome and of its activator Cdc20 in functional substrate binding.
Miniowitz-Shemtov S, Teichner A, Sitry-Shevah D, Hershko A
Proceedings of the National Academy of Sciences of the United States of America 2010 Mar 23;107(12):5351-6
Proceedings of the National Academy of Sciences of the United States of America 2010 Mar 23;107(12):5351-6
Identification of novel human Cdt1-binding proteins by a proteomics approach: proteolytic regulation by APC/CCdh1.
Sugimoto N, Kitabayashi I, Osano S, Tatsumi Y, Yugawa T, Narisawa-Saito M, Matsukage A, Kiyono T, Fujita M
Molecular biology of the cell 2008 Mar;19(3):1007-21
Molecular biology of the cell 2008 Mar;19(3):1007-21
Inhibitory factors associated with anaphase-promoting complex/cylosome in mitotic checkpoint.
Braunstein I, Miniowitz S, Moshe Y, Hershko A
Proceedings of the National Academy of Sciences of the United States of America 2007 Mar 20;104(12):4870-5
Proceedings of the National Academy of Sciences of the United States of America 2007 Mar 20;104(12):4870-5
Roles of the anaphase-promoting complex/cyclosome and of its activator Cdc20 in functional substrate binding.
Eytan E, Moshe Y, Braunstein I, Hershko A
Proceedings of the National Academy of Sciences of the United States of America 2006 Feb 14;103(7):2081-6
Proceedings of the National Academy of Sciences of the United States of America 2006 Feb 14;103(7):2081-6
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- A-431
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 55
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human lymph node shows strong cytoplasmic and nuclear positivity in reaction center cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
