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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [4]
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- Product number
- NB100-59828 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-59828, RRID:AB_892464
- Product name
- Rabbit Polyclonal Cdc20 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Regulation of OLC1 protein expression by the anaphase-promoting complex.
Unliganded progesterone receptors attenuate taxane-induced breast cancer cell death by modulating the spindle assembly checkpoint.
Cdc20 mediates D-box-dependent degradation of Sp100.
How cyclin A destruction escapes the spindle assembly checkpoint.
Zhang X, Chen W, Yin N, Dong L, Fu M, Zhan Q, Tong T
Oncology letters 2019 Mar;17(3):2639-2646
Oncology letters 2019 Mar;17(3):2639-2646
Unliganded progesterone receptors attenuate taxane-induced breast cancer cell death by modulating the spindle assembly checkpoint.
Badtke MM, Jambal P, Dye WW, Spillman MA, Post MD, Horwitz KB, Jacobsen BM
Breast cancer research and treatment 2012 Jan;131(1):75-87
Breast cancer research and treatment 2012 Jan;131(1):75-87
Cdc20 mediates D-box-dependent degradation of Sp100.
Wang R, Li KM, Zhou CH, Xue JL, Ji CN, Chen JZ
Biochemical and biophysical research communications 2011 Dec 2;415(4):702-6
Biochemical and biophysical research communications 2011 Dec 2;415(4):702-6
How cyclin A destruction escapes the spindle assembly checkpoint.
Di Fiore B, Pines J
The Journal of cell biology 2010 Aug 23;190(4):501-9
The Journal of cell biology 2010 Aug 23;190(4):501-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human CDC20 on HeLa whole cell lysate using NB100-59828. CDC20 was also immunoprecipitated by rabbit anti-CDC20 antibody NB100-59827.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cdc20 Antibody [NB100-59828] - Detection of Human and Mouse CDC20 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody NB100-59828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Cdc20 Antibody [NB100-59828] - Detection of human CDC20 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDC20 antibody NB100-59828 (lot NB100-59828-2) used for IP at 6 ug per reaction. CDC20 was also immunoprecipitated by a previous lot of this antibody (lot NB100-59828-1) and rabbit anti-CDC20 antibody NB100-59827. For blotting immunoprecipitated CDC20, NB100-59828 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] - Human breast carcinoma (left) and mouse teratoma (right). Antibody used at a dilution of 1:1,000 (0.2ug/ml).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:250.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59828] - Section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB.
