H00008850-M04

antibody from Abnova Corporation

Targeting: KAT2B GCN5, GCN5L, P/CAF, PCAF

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008850-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00008850-M04, RRID:AB_733233

Product name

PCAF monoclonal antibody (M04), clone 5E11

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant PCAF.

Antigen sequence

LEEEVYSQNSPIWDQDFLSASSRTSQLGIQTVINP
PPVAGTISYNSTSSSLEQPNAGSSSPACKASSGLE
ANPGEKRKMTDSHVLEEAKKPRVMGDIPME


Isotype

IgG

Antibody clone number

5E11

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PCAF monoclonal antibody (M04), clone 5E11. Western Blot analysis of PCAF expression in human uterus myoma.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PCAF monoclonal antibody (M04), clone 5E11. Western Blot analysis of PCAF expression in A-431 ( Cat # L015V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PCAF monoclonal antibody (M04), clone 5E11. Western Blot analysis of PCAF expression in LNCaP ( Cat # L004V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PCAF is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to PCAF on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol