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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-38547 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCAF Monoclonal Antibody (7D12C8)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- This antibody has been tested in indirect ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7D12C8
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis using PCAF Monoclonal Antibody (Product # MA5-38547) against human KAT2B (AA: 1-142) recombinant protein. (Expected MW is 39.3 kDa)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PCAF using PCAF Monoclonal Antibody (Product # MA5-38547) in HEK293 (1) and KAT2B (AA: 1-142)-human IgG-Fc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of PCAF in HeLa cells using PCAF Monoclonal Antibody (Product # MA5-38547) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PCAF in paraffin-embedded cervical cancer tissues using PCAF Monoclonal Antibody (Product # MA5-38547) and DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PCAF in paraffin-embedded rectum cancer tissues using PCAF Monoclonal Antibody (Product # MA5-38547) and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of PCAF in MOLT4 cells using PCAF Monoclonal Antibody (Product # MA5-38547) (green) and negative control (red).
