AP53342PU-N

antibody from Acris Antibodies GmbH

Targeting: PLD1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP53342PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP53342PU-N, RRID:AB_11145563

Product name

anti Phospholipase D1 / PLD1 (C-term)

Antibody type

Polyclonal

Antigen

KLH conjugated synthetic peptide between 1018-1049 amino acids from the C-terminal region of human Phospholipase D1 / PLD1

Reactivity

Human

Host

Rabbit

Vial size

0.1 mg

Concentration

0.25 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of PLD1 (arrow) using Phospholipase D1 / PLD1 Antibody (C-term) Cat.-No AP53342PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PLD1 gene.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue reacted with Phospholipase D1 / PLD1 Antibody (C-term) Cat.-No AP53342PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining.This data demonstrates the use of PLD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Flow cytometric analysis of 293 cells using Phospholipase D1 / PLD1 Antibody (C-term) Cat.-No AP53342PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis