Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 649201 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#649201, RRID:AB_10613283
- Product name
- Purified anti-GAPDH
- Antibody type
- Monoclonal
- Antigen
- Human CD4 lymphocytes
- Description
- This mouse monoclonal GAPDH antibody recognizes human GAPDH, also known as glyceraldehyde-3-phosphate dehydrogenase. GAPDH is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. GAPDH is a ubiquitously expressed and has a molecular mass of 36 kD. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. In more recent studies, GAPDH has been shown to be involved in microtubule bundling, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases. The FF26A/F9 monoclonal antibody has been shown to be useful for Western blotting.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- Upon receipt, store undiluted at 4°C.
Submitted references Fibronectin induces endothelial cell migration through β1 integrin and Src-dependent phosphorylation of fibroblast growth factor receptor-1 at tyrosines 653/654 and 766.
Zou L, Cao S, Kang N, Huebert RC, Shah VH
The Journal of biological chemistry 2012 Mar 2;287(10):7190-202
The Journal of biological chemistry 2012 Mar 2;287(10):7190-202
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- HeLa cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-GAPDH antibody (clone FF26A/F9). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated