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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA328750 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-TRPP1 (PKD2)
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-TRPP1 (PKD2)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PKD2
- Antibody clone number
- NULL
- Vial size
- 200 µl
- Concentration
- NULL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of rat kidney membrane proteins (lanes 1 and 3) and mouse kidney lysate (lanes 2 and 4): 1, 2. Anti-TRPP1 (PKD2) antibody, (1:200). 3, 4. Anti-TRPP1 (PKD2) antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Expression of TRPP1 (PKD2) in C2C12 cell line. Immunocytochemical staining of TRPP1 (PKD2) in mouse paraformaldehyde-fixed and permeabilized muscle myoblasts (C2C12) cell line. A. Cells were stained with Anti-TRPP1 (PKD2) antibody, (1:50-1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged images of panels A and B.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IHC staining of rat kidney paraffin embedded sections using Anti-TRPP1 (PKD2) antibody, (1:100), followed by goat anti-rabbit-Alexa-Fluor-594 secondary antibody. A. TRPP1 staining followed by goat anti-rabbit-Alexa-Fluor-594 secondary antibody (red) appears in the renal cortex, mainly in the distal tubules. B. DAB staining of TRPP1 (brown) also shows that the renal corpuscle and the blood vessels are not stained at all. (PT=proximal tubule, DT = distal tubule).
- Validation comment
- IHC
