Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP32809PU-N - Provider product page
- Provider
- OriGene
- Product name
- PRAK (MAPKAPK5) (192-206) goat polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- PRAK (MAPKAPK5) (192-206) goat polyclonal antibody, Aff - Purified
- Host
- Goat
- Conjugate
- Unconjugated
- Epitope
- MAPKAPK5
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293 lysate (10ug protein in RIPA?buffer) overexpressing Mouse MK5-GFP?(first lane) or Mouse MK2-GFP (second?lane) probed with -N (0.5?ug/ml) in right panel and with? (0.5ug/ml) on left panel,?Primary incubations were for 2 hours.?Detected by chemiluminescence.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- -N and-N (1.5 ug)?immunoprecipitations from lysates of?MK5/ERK3 double knockout MEFs, with?(third and fifth lanes) and without (fourth?and sixth lanes) rescued MK5/ERK3?expression through retroviral?transduction. The corresponding lysates?(first an
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ?(5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- IHC