PAB0520

antibody from Abnova Corporation

Targeting: MAPKAPK5 MK5, PRAK

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB0520

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB0520, RRID:AB_982823

Product name

MAPKAPK5 (phospho S93) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAPKAPK5.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

PRAK, a novel protein kinase regulated by the p38 MAP kinase.

New L, Jiang Y, Zhao M, Liu K, Zhu W, Flood LJ, Kato Y, Parry GC, Han J
The EMBO journal 1998 Jun 15;17(12):3372-84

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MAPKAPK5, a novel mitogen-activated protein kinase (MAPK)-activated protein kinase, is a substrate of the extracellular-regulated kinase (ERK) and p38 kinase.

Ni H, Wang XS, Diener K, Yao Z
Biochemical and biophysical research communications 1998 Feb 13;243(2):492-6

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

The MAPKAPK5 (phospho S93) polyclonal antibody (Cat # PAB0520) is used in Western blot to detect Phospho-MAPKAPK5-S93 in mouse heart tissue lysate. PAD4 (arrow) was detected using the purified polyclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MAPKAPK5 (phospho S93) polyclonal antibody (Cat # PAB0520) which was peroxidase-conjugated to the secondary antibody followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)