16476-1-AP

antibody from Invitrogen Antibodies

Targeting: JMJD6 KIAA0585, PTDSR, PTDSR1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16476-1-AP

Provider

Invitrogen Antibodies

Product name

JMJD6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MNHKSKKRI REAKRSARPE LKDSLDWTRH NYYESFSLSP AAVADNVERA DALQLSVEEF VERYERPYKP VVLLNAQEGW SAQEKWTLER LKRKYRNQKF KCGEDNDGYS VKMKMKYYIE YMESTRDDSP LYIFDSSYGE HPKRRKLLED YKVPKFFTDD LFQYAGEKRR PPYRWFVMGP PRSGTGIHID PLGTSAWNAL VQGHKRWCLF PTSTPRELIK VTRDEGGNQQ DEAITWFNVI YPRTQLPTWP PEFKPLEILQ KPGETVFVPG GWWHVVLNLD TTIAITQNFA SSTNFPVVWH KTVRGRPKLS RKWYRILKQE HPELAVLADS VDLQESTGIA SDSSSDSSSS SSSSSSDSDS ECESGSEGDG TVHRRKKRRT CSMVGNGDTT SQDDCVSKER SSSR (1-403 aa encoded by BC066654)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.37 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 16476-1-AP (JMJD6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart using 16476-1-AP (JMJD6 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart using 16476-1-AP (JMJD6 antibody) at dilution of 1:50 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 K-562 cells were stained with 0.2ug JMJD6 antibody (16476-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-JMJD6 (IP:16476-1-AP, 4ug; Detection:16476-1-AP 1:500) with K-562 cells lysate 1200ug.