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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-8048 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-8048, RRID:AB_628219
- Product name
- Anti-RHOB
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Mouse
Submitted references ERK1/2 signaling dominates over RhoA signaling in regulating early changes in RNA expression induced by endothelin-1 in neonatal rat cardiomyocytes.
The C-terminal sequence of RhoB directs protein degradation through an endo-lysosomal pathway.
The role of geranylgeranylated proteins in human mesangial cell proliferation
Marshall AK, Barrett OP, Cullingford TE, Shanmugasundram A, Sugden PH, Clerk A
PloS one 2010 Apr 2;5(4):e10027
PloS one 2010 Apr 2;5(4):e10027
The C-terminal sequence of RhoB directs protein degradation through an endo-lysosomal pathway.
Pérez-Sala D, Boya P, Ramos I, Herrera M, Stamatakis K
PloS one 2009 Dec 2;4(12):e8117
PloS one 2009 Dec 2;4(12):e8117
The role of geranylgeranylated proteins in human mesangial cell proliferation
A Khwaja, C C Sharpe, M Noor, B M Hendry
Kidney International 2006 Aug;70(7):1296-1304
Kidney International 2006 Aug;70(7):1296-1304
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 8
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
