sc-360

antibody from Santa Cruz Biotechnology

Targeting: ARHGDIA GDIA1, RHOGDI

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-360

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-360, RRID:AB_2227516

Product name

Anti-ARHGDIA

Antibody type

Polyclonal (Antigen purified)

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

References

Submitted references

The effects of deep brain stimulation on sleep in Parkinson's disease.

Amara AW, Watts RL, Walker HC
Therapeutic advances in neurological disorders 2011 Jan;4(1):15-24

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Geranylgeranyltransferase type I (GGTase-I) deficiency hyperactivates macrophages and induces erosive arthritis in mice.

Khan OM, Ibrahim MX, Jonsson IM, Karlsson C, Liu M, Sjogren AK, Olofsson FJ, Brisslert M, Andersson S, Ohlsson C, Hultén LM, Bokarewa M, Bergo MO
The Journal of clinical investigation 2011 Feb;121(2):628-39

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Peroxisome proliferator-activated receptor beta activation promotes myonuclear accretion in skeletal muscle of adult and aged mice.

Giordano C, Rousseau AS, Wagner N, Gaudel C, Murdaca J, Jehl-Piétri C, Sibille B, Grimaldi PA, Lopez P
Pflugers Archiv : European journal of physiology 2009 Sep;458(5):901-13

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Activation of host translational control pathways by a viral developmental switch.

Arias C, Walsh D, Harbell J, Wilson AC, Mohr I
PLoS pathogens 2009 Mar;5(3):e1000334

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Lack of nuclear translocation of cytoplasmic domains of IL-2/IL-15 receptor subunits.

Fujii H, Hoshino A
Cytokine 2009 Jun;46(3):302-8

---

Morphological and proliferative abnormalities in renal mesangial cells lacking RhoGDI.

Bielek H, Anselmo A, Dermardirossian C
Cellular signalling 2009 Dec;21(12):1974-83

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The C-terminal sequence of RhoB directs protein degradation through an endo-lysosomal pathway.

Pérez-Sala D, Boya P, Ramos I, Herrera M, Stamatakis K
PloS one 2009 Dec 2;4(12):e8117

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Effects of lovastatin on Rho isoform expression, activity, and association with guanine nucleotide dissociation inhibitors.

Turner SJ, Zhuang S, Zhang T, Boss GR, Pilz RB
Biochemical pharmacology 2008 Jan 15;75(2):405-13

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Inhibitors of protein geranylgeranyltransferase I and Rab geranylgeranyltransferase identified from a library of allenoate-derived compounds.

Watanabe M, Fiji HD, Guo L, Chan L, Kinderman SS, Slamon DJ, Kwon O, Tamanoi F
The Journal of biological chemistry 2008 Apr 11;283(15):9571-9

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High-throughput identification of IMCD proteins using LC-MS/MS.

Pisitkun T, Bieniek J, Tchapyjnikov D, Wang G, Wu WW, Shen RF, Knepper MA
Physiological genomics 2006 Apr 13;25(2):263-76

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band corresponding to the predicted size in kDa (+/-20%).

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 229, 107, 78, 46, 31, 26, 16.2

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 23

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.

Validation comment

Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.