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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [21]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-553 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-553, RRID:AB_2184874
- Product name
- Anti-RXRA
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references Cathepsin H regulated by the thyroid hormone receptors associate with tumor invasion in human hepatoma cells
Functional significance of vitamin D receptor FokI polymorphism in human breast cancer cells.
Anti-tumor effects of retinoids combined with trastuzumab or tamoxifen in breast cancer cells: induction of apoptosis by retinoid/trastuzumab combinations.
p27(Kip1) deficiency promotes prostate carcinogenesis but does not affect the efficacy of retinoids in suppressing the neoplastic process.
Proteasomal degradation of retinoid X receptor alpha reprograms transcriptional activity of PPARgamma in obese mice and humans.
Epigenetic regulatory mechanisms distinguish retinoic acid-mediated transcriptional responses in stem cells and fibroblasts.
Long chain acyl-CoA synthetase-3 is a molecular target for peroxisome proliferator-activated receptor delta in HepG2 hepatoma cells.
Retinoid X receptor alpha controls innate inflammatory responses through the up-regulation of chemokine expression.
Protein kinase Cbeta mediates hepatic induction of sterol-regulatory element binding protein-1c by insulin.
Cooperative interaction between retinoic acid receptor-alpha and estrogen receptor in breast cancer.
Peroxisome proliferator-activated receptor gamma regulates expression of the anti-lipolytic G-protein-coupled receptor 81 (GPR81/Gpr81).
Infection decreases fatty acid oxidation and nuclear hormone receptors in the diaphragm.
Control of TCF-4 expression by VDR and vitamin D in the mouse mammary gland and colorectal cancer cell lines.
Identification of an inter-transcription factor regulatory network in human hepatoma cells by Matrix RNAi.
Rexinoid-induced expression of IGFBP-6 requires RARbeta-dependent permissive cooperation of retinoid receptors and AP-1.
Regulation of gene expression of hepatic drug metabolizing enzymes and transporters by the Toll-like receptor 2 ligand, lipoteichoic acid.
Cutting edge: Inhibition of NF-kappaB-mediated TSLP expression by retinoid X receptor.
Transactivation of the hepatitis B virus core promoter by the nuclear receptor FXRalpha.
PPARgamma and C/EBP factors orchestrate adipocyte biology via adjacent binding on a genome-wide scale.
Activation of estrogen signaling pathways collaborates with loss of Brca1 to promote development of ERalpha-negative and ERalpha-positive mammary preneoplasia and cancer.
Retinoid regulated association of transcriptional co-regulators and the polycomb group protein SUZ12 with the retinoic acid response elements of Hoxa1, RARbeta(2), and Cyp26A1 in F9 embryonal carcinoma cells.
S-M Wu, Y-H Huang, C-T Yeh, M-M Tsai, C-H Liao, W-L Cheng, W-J Chen, K-H Lin
Oncogene 2011 Jan;30(17):2057-2069
Oncogene 2011 Jan;30(17):2057-2069
Functional significance of vitamin D receptor FokI polymorphism in human breast cancer cells.
Alimirah F, Peng X, Murillo G, Mehta RG
PloS one 2011 Jan 24;6(1):e16024
PloS one 2011 Jan 24;6(1):e16024
Anti-tumor effects of retinoids combined with trastuzumab or tamoxifen in breast cancer cells: induction of apoptosis by retinoid/trastuzumab combinations.
Koay DC, Zerillo C, Narayan M, Harris LN, DiGiovanna MP
Breast cancer research : BCR 2010;12(4):R62
Breast cancer research : BCR 2010;12(4):R62
p27(Kip1) deficiency promotes prostate carcinogenesis but does not affect the efficacy of retinoids in suppressing the neoplastic process.
Taylor W, Mathias A, Ali A, Ke H, Stoynev N, Shilkaitis A, Green A, Kiyokawa H, Christov K
BMC cancer 2010 Oct 8;10:541
BMC cancer 2010 Oct 8;10:541
Proteasomal degradation of retinoid X receptor alpha reprograms transcriptional activity of PPARgamma in obese mice and humans.
Lefebvre B, Benomar Y, Guédin A, Langlois A, Hennuyer N, Dumont J, Bouchaert E, Dacquet C, Pénicaud L, Casteilla L, Pattou F, Ktorza A, Staels B, Lefebvre P
The Journal of clinical investigation 2010 May;120(5):1454-68
The Journal of clinical investigation 2010 May;120(5):1454-68
Epigenetic regulatory mechanisms distinguish retinoic acid-mediated transcriptional responses in stem cells and fibroblasts.
Kashyap V, Gudas LJ
The Journal of biological chemistry 2010 May 7;285(19):14534-48
The Journal of biological chemistry 2010 May 7;285(19):14534-48
Long chain acyl-CoA synthetase-3 is a molecular target for peroxisome proliferator-activated receptor delta in HepG2 hepatoma cells.
Cao A, Li H, Zhou Y, Wu M, Liu J
The Journal of biological chemistry 2010 May 28;285(22):16664-74
The Journal of biological chemistry 2010 May 28;285(22):16664-74
Retinoid X receptor alpha controls innate inflammatory responses through the up-regulation of chemokine expression.
Núñez V, Alameda D, Rico D, Mota R, Gonzalo P, Cedenilla M, Fischer T, Boscá L, Glass CK, Arroyo AG, Ricote M
Proceedings of the National Academy of Sciences of the United States of America 2010 Jun 8;107(23):10626-31
Proceedings of the National Academy of Sciences of the United States of America 2010 Jun 8;107(23):10626-31
Protein kinase Cbeta mediates hepatic induction of sterol-regulatory element binding protein-1c by insulin.
Yamamoto T, Watanabe K, Inoue N, Nakagawa Y, Ishigaki N, Matsuzaka T, Takeuchi Y, Kobayashi K, Yatoh S, Takahashi A, Suzuki H, Yahagi N, Gotoda T, Yamada N, Shimano H
Journal of lipid research 2010 Jul;51(7):1859-70
Journal of lipid research 2010 Jul;51(7):1859-70
Cooperative interaction between retinoic acid receptor-alpha and estrogen receptor in breast cancer.
Ross-Innes CS, Stark R, Holmes KA, Schmidt D, Spyrou C, Russell R, Massie CE, Vowler SL, Eldridge M, Carroll JS
Genes & development 2010 Jan 15;24(2):171-82
Genes & development 2010 Jan 15;24(2):171-82
Peroxisome proliferator-activated receptor gamma regulates expression of the anti-lipolytic G-protein-coupled receptor 81 (GPR81/Gpr81).
Jeninga EH, Bugge A, Nielsen R, Kersten S, Hamers N, Dani C, Wabitsch M, Berger R, Stunnenberg HG, Mandrup S, Kalkhoven E
The Journal of biological chemistry 2009 Sep 25;284(39):26385-93
The Journal of biological chemistry 2009 Sep 25;284(39):26385-93
Infection decreases fatty acid oxidation and nuclear hormone receptors in the diaphragm.
Feingold KR, Moser A, Patzek SM, Shigenaga JK, Grunfeld C
Journal of lipid research 2009 Oct;50(10):2055-63
Journal of lipid research 2009 Oct;50(10):2055-63
Control of TCF-4 expression by VDR and vitamin D in the mouse mammary gland and colorectal cancer cell lines.
Beildeck ME, Islam M, Shah S, Welsh J, Byers SW
PloS one 2009 Nov 17;4(11):e7872
PloS one 2009 Nov 17;4(11):e7872
Identification of an inter-transcription factor regulatory network in human hepatoma cells by Matrix RNAi.
Tomaru Y, Nakanishi M, Miura H, Kimura Y, Ohkawa H, Ohta Y, Hayashizaki Y, Suzuki M
Nucleic acids research 2009 Mar;37(4):1049-60
Nucleic acids research 2009 Mar;37(4):1049-60
Rexinoid-induced expression of IGFBP-6 requires RARbeta-dependent permissive cooperation of retinoid receptors and AP-1.
Uray IP, Shen Q, Seo HS, Kim H, Lamph WW, Bissonnette RP, Brown PH
The Journal of biological chemistry 2009 Jan 2;284(1):345-353
The Journal of biological chemistry 2009 Jan 2;284(1):345-353
Regulation of gene expression of hepatic drug metabolizing enzymes and transporters by the Toll-like receptor 2 ligand, lipoteichoic acid.
Ghose R, Guo T, Haque N
Archives of biochemistry and biophysics 2009 Jan 1;481(1):123-30
Archives of biochemistry and biophysics 2009 Jan 1;481(1):123-30
Cutting edge: Inhibition of NF-kappaB-mediated TSLP expression by retinoid X receptor.
Lee HC, Headley MB, Iseki M, Ikuta K, Ziegler SF
Journal of immunology (Baltimore, Md. : 1950) 2008 Oct 15;181(8):5189-93
Journal of immunology (Baltimore, Md. : 1950) 2008 Oct 15;181(8):5189-93
Transactivation of the hepatitis B virus core promoter by the nuclear receptor FXRalpha.
Ramière C, Scholtès C, Diaz O, Icard V, Perrin-Cocon L, Trabaud MA, Lotteau V, André P
Journal of virology 2008 Nov;82(21):10832-40
Journal of virology 2008 Nov;82(21):10832-40
PPARgamma and C/EBP factors orchestrate adipocyte biology via adjacent binding on a genome-wide scale.
Lefterova MI, Zhang Y, Steger DJ, Schupp M, Schug J, Cristancho A, Feng D, Zhuo D, Stoeckert CJ Jr, Liu XS, Lazar MA
Genes & development 2008 Nov 1;22(21):2941-52
Genes & development 2008 Nov 1;22(21):2941-52
Activation of estrogen signaling pathways collaborates with loss of Brca1 to promote development of ERalpha-negative and ERalpha-positive mammary preneoplasia and cancer.
Jones LP, Tilli MT, Assefnia S, Torre K, Halama ED, Parrish A, Rosen EM, Furth PA
Oncogene 2008 Jan 31;27(6):794-802
Oncogene 2008 Jan 31;27(6):794-802
Retinoid regulated association of transcriptional co-regulators and the polycomb group protein SUZ12 with the retinoic acid response elements of Hoxa1, RARbeta(2), and Cyp26A1 in F9 embryonal carcinoma cells.
Gillespie RF, Gudas LJ
Journal of molecular biology 2007 Sep 14;372(2):298-316
Journal of molecular biology 2007 Sep 14;372(2):298-316
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 220, 112, 84, 47, 32, 26, 16.8
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 51
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
