Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AB1024-200 - Provider product page
- Provider
- SICGEN
- Proper citation
- SICGEN Cat#AB1024-200, RRID:AB_2333166
- Product name
- Anti-Rab5
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody to mouse Rab5a. Rab5a belongs to the small GTPase superfamily, Rab family. Rab5a is an Early Endosome Marker and functions as a key regulator of vesicular trafficking during early endocytosis.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 600 µg
- Concentration
- 3 mg/ml
- Storage
- Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Anti-Rab5 Ab (AB1024) at 1/1,000 dilution; tissue lysate at 100 µg per lane
- Sample type
- Spleen
- Primary Ab dilution
- 1/1,000
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1/10,000
Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Immunofluorescence – anti-Rab5 Ab (AB1024) using B6-RPE07 cells at 1/50 dilution; cells were fixed with 4% of PFA;
- Sample type
- B6-RPE07 cells
- Primary Ab dilution
- 1/50
- Conjugate
- Unconjugated