Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF-455-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse SCF/c-kit Ligand Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse SCF/c-kit Ligand in ELISAs and Western blots. In Western blots, less than 20% cross-reactivity with recombinant human SCF is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q78ED8
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Response patterns of cytokines/chemokines in two murine strains after irradiation.
Role of stem cell factor and bone marrow-derived fibroblasts in airway remodeling.
Steel factor controls primordial germ cell survival and motility from the time of their specification in the allantois, and provides a continuous niche throughout their migration.
SCF-mediated mast cell infiltration and activation exacerbate the inflammation and immunosuppression in tumor microenvironment.
Nociceptive tuning by stem cell factor/c-Kit signaling.
Indispensable role of Bcl2 in the development of the melanocyte stem cell.
The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine 2012 May;58(2):169-77
Cytokine 2012 May;58(2):169-77
Role of stem cell factor and bone marrow-derived fibroblasts in airway remodeling.
Dolgachev VA, Ullenbruch MR, Lukacs NW, Phan SH
The American journal of pathology 2009 Feb;174(2):390-400
The American journal of pathology 2009 Feb;174(2):390-400
Steel factor controls primordial germ cell survival and motility from the time of their specification in the allantois, and provides a continuous niche throughout their migration.
Gu Y, Runyan C, Shoemaker A, Surani A, Wylie C
Development (Cambridge, England) 2009 Apr;136(8):1295-303
Development (Cambridge, England) 2009 Apr;136(8):1295-303
SCF-mediated mast cell infiltration and activation exacerbate the inflammation and immunosuppression in tumor microenvironment.
Huang B, Lei Z, Zhang GM, Li D, Song C, Li B, Liu Y, Yuan Y, Unkeless J, Xiong H, Feng ZH
Blood 2008 Aug 15;112(4):1269-79
Blood 2008 Aug 15;112(4):1269-79
Nociceptive tuning by stem cell factor/c-Kit signaling.
Milenkovic N, Frahm C, Gassmann M, Griffel C, Erdmann B, Birchmeier C, Lewin GR, Garratt AN
Neuron 2007 Dec 6;56(5):893-906
Neuron 2007 Dec 6;56(5):893-906
Indispensable role of Bcl2 in the development of the melanocyte stem cell.
Mak SS, Moriyama M, Nishioka E, Osawa M, Nishikawa S
Developmental biology 2006 Mar 1;291(1):144-53
Developmental biology 2006 Mar 1;291(1):144-53
The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M
Archives of dermatological research 2001 Nov;293(10):508-14
Archives of dermatological research 2001 Nov;293(10):508-14
The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M
Archives of dermatological research 2001 Nov;293(10):508-14
Archives of dermatological research 2001 Nov;293(10):508-14
A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
Shih CC, Hu MC, Hu J, Weng Y, Yazaki PJ, Medeiros J, Forman SJ
Blood 2000 Mar 15;95(6):1957-66
Blood 2000 Mar 15;95(6):1957-66
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by SCF/c-kit Ligand and Neutralization by Mouse SCF/c-kit Ligand Antibody. Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC) stimulates prolif-eration in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse SCF/c-kit Ligand (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse SCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-455-NA). The ND50 is typically 0.3-0.6 µg/mL.