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LearnNBP1-77030
antibody from Novus Biologicals
Targeting: KITLG
DFNA69, FPH2, Kitl, KL-1, MGF, SCF, SF, SLF
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [7]
- Immunohistochemistry [1]
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- Product number
- NBP1-77030 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77030, RRID:AB_11039334
- Product name
- Rabbit Polyclonal SCF/c-kit Ligand Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Targeting kit activation: a potential therapeutic approach in the treatment of allergic inflammation.
Targeting kit activation: a potential therapeutic approach in the treatment of allergic inflammation.
Stem cell factor and its receptor c-Kit as targets for inflammatory diseases.
Stem cell factor (c-kit ligand) influences eosinophil recruitment and histamine levels in allergic airway inflammation.
Jensen BM, Metcalfe DD, Gilfillan AM
Inflammation & allergy drug targets 2007 Mar;6(1):57-62
Inflammation & allergy drug targets 2007 Mar;6(1):57-62
Targeting kit activation: a potential therapeutic approach in the treatment of allergic inflammation.
Jensen BM, Metcalfe DD, Gilfillan AM
Inflammation & allergy drug targets 2007 Mar;6(1):57-62
Inflammation & allergy drug targets 2007 Mar;6(1):57-62
Stem cell factor and its receptor c-Kit as targets for inflammatory diseases.
Reber L, Da Silva CA, Frossard N
European journal of pharmacology 2006 Mar 8;533(1-3):327-40
European journal of pharmacology 2006 Mar 8;533(1-3):327-40
Stem cell factor (c-kit ligand) influences eosinophil recruitment and histamine levels in allergic airway inflammation.
Lukacs NW, Strieter RM, Lincoln PM, Brownell E, Pullen DM, Schock HJ, Chensue SW, Taub DD, Kunkel SL
Journal of immunology (Baltimore, Md. : 1950) 1996 May 15;156(10):3945-51
Journal of immunology (Baltimore, Md. : 1950) 1996 May 15;156(10):3945-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Analysis of SCF in A431 cell lysate with SCF antibody at (A) 1 and (B) 2 ug/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Analysis of SCF in A431 cell lysate with SCF antibody at (A) 1 and (B) 2 ug/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Loading: 15 ug of lysates per lane. Antibodies: SCF (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Loading: 30 ng of human SCF recombinant protein per lane. Antibodies: SCF (Lane 1: 1 ug/mL and Lane 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 20kD.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - ISOFORMS: Human SCF has 3 isoforms, including isoform 1 (273aa, 30.9kD), isoform 2 (245aa, 27.9kD) and isoform 3 (238aa, 26.7kD). Mouse SCF has 2 isoforms, including isoform 1 (273aa, 30.6kD) and isoform 2 (245aa, 27.6kD). Rat SCF also has 2 isoforms, including isoform 1 (273aa, 30.7kD) and isoform 2 (245aa, 27.7kD). This antibody can detect human and possibly detect mouse and rat.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - WB analysis showed protein expression level of SCF detected by anti-SCF antibody in gastric smooth muscle was significantly decreased in STZ-induced diabetic mice as compared to the control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Analysis of protein expression level of SCF detected by anti-SCF antibody. GSMCs were treated with C-type natriuretic peptide (CNP) at different doses for 48hr. CNP(10^-7mol/L and 10^-6mol/L) significantly decreased SCF protein expression level as compared to the control group.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SCF/c-kit Ligand Antibody [NBP1-77030] - Mouse brain tissue with SCF antibody at 2.5 ug/ml.
