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LearnPA5-19793
antibody from Invitrogen Antibodies
Targeting: KITLG
DFNA69, FPH2, Kitl, KL-1, MGF, SCF, SF, SLF
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunohistochemistry [1]
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- Product number
- PA5-19793 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SCF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For Western Blot, this antibody has non-specific bands at 120 kDa and 50 kDa. Heat mediated antigen retrieval recommended prior to tissue staining. This antibody is predicted to react with rat, sheep, goat, horse, cow, cat, dog, pig and rhesus monkey based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical (formalin-fixed, paraffin-embedded) staining of Human Normal Colon tissue using Product # PA5-19793, anti-SCF antibody. Primary antibody was used at a concentration of 5 µg/mL and exposed for 15 mins at room temp. The sample was pretreated using heat mediated antigen retrieval with Sodium Citrate Buffer (pH6/20mins). The detection method was a HRP conjugated polymer, DAB chromogen and the sample was counterstained with haematoxylin and mounted with DPX.
