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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- R32075 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Surfactant protein D Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids RSAAENAALQQLVVAKNEAAFLSMTD SKTE of human Surfactant protein D were used as the immunogen for the Surfactant protein D antibody.
- Description
- Antigen affinity
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the Surfactant protein D antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat lung, 2) rat brain and 3) human PANC lysate with Surfactant protein D antibody. Predicted/observed molecular weight ~38 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of FFPE human HeLa cells with Surfactant protein D antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of FFPE human A549 cells with Surfactant protein D antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human lung cancer tissue with Surfactant protein D antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of frozen human placental tissue with Surfactant protein D antibody.
