AF1920

antibody from R&D Systems

Targeting: SFTPD COLEC7, SFTP4, SP-D

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1920

Provider

R&D Systems

Product name

Human/Mouse/Rat SP-D Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human SP-D in direct ELISAs and human, mouse, and rat SP-D in Western blots.

Reactivity

Human, Mouse, Rat

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P35247


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Oxidative damage of SP-D abolishes control of eosinophil extracellular DNA trap formation.

Yousefi S, Sharma SK, Stojkov D, Germic N, Aeschlimann S, Ge MQ, Flayer CH, Larson ED, Redai IG, Zhang S, Koziol-White CJ, Karikó K, Simon HU, Haczku A
Journal of leukocyte biology 2018 Jul;104(1):205-214

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MMP-9 cleaves SP-D and abrogates its innate immune functions in vitro.

Bratcher PE, Weathington NM, Nick HJ, Jackson PL, Snelgrove RJ, Gaggar A
PloS one 2012;7(7):e41881

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Mouse SP-D by Simple Western<SUP abp="246">TM. Simple Western lane view shows lysates of human lung tissue and mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for SP-D at approximately 64/59 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat SP-D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1920) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human, Mouse, and Rat SP-D by Western Blot. Western blot shows lysates of human lung tissue, mouse lung tissue, and rat lung tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat SP-D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1920) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SP-D at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.