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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-17800 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-17800, RRID:AB_2202719
- Product name
- Anti-TGIF
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references Smad ubiquitination regulatory factor-2 in the fibrotic kidney: regulation, target specificity, and functional implication.
hepatocyte growth factor is a downstream effector that mediates the antifibrotic action of peroxisome proliferator-activated receptor-gamma agonists.
Hepatocyte growth factor receptor signaling mediates the anti-fibrotic action of 9-cis-retinoic acid in glomerular mesangial cells.
Tan R, He W, Lin X, Kiss LP, Liu Y
American journal of physiology. Renal physiology 2008 May;294(5):F1076-83
American journal of physiology. Renal physiology 2008 May;294(5):F1076-83
hepatocyte growth factor is a downstream effector that mediates the antifibrotic action of peroxisome proliferator-activated receptor-gamma agonists.
Li Y, Wen X, Spataro BC, Hu K, Dai C, Liu Y
Journal of the American Society of Nephrology : JASN 2006 Jan;17(1):54-65
Journal of the American Society of Nephrology : JASN 2006 Jan;17(1):54-65
Hepatocyte growth factor receptor signaling mediates the anti-fibrotic action of 9-cis-retinoic acid in glomerular mesangial cells.
Wen X, Li Y, Hu K, Dai C, Liu Y
The American journal of pathology 2005 Oct;167(4):947-57
The American journal of pathology 2005 Oct;167(4):947-57
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 28
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
