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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-34685 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TrxR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2.
- Concentration
- 1 mg/mL
Submitted references Expression of TXNRD1, HSPA4L and ATP1B1 Genes Associated with the Freezability of Boar Sperm.
Mańkowska A, Gilun P, Zasiadczyk Ł, Sobiech P, Fraser L
International journal of molecular sciences 2022 Aug 18;23(16)
International journal of molecular sciences 2022 Aug 18;23(16)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TrxR1 Polyclonal Antibody (Product # PA5-34685). Sample (30 µg of whole cell lysate). A: Hep G2. 7.5% SDS PAGE. TrxR1 Polyclonal Antibody (Product # PA5-34685) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TrxR1 Polyclonal Antibody detects TrxR1 protein at nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TrxR1 protein stained by TrxR1 Polyclonal Antibody (Product # PA5-34685) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded N87 xenograft, using TXNRD1 (Product # PA5-34685) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TrxR1 Polyclonal Antibody detects TrxR1 protein at cytoplasm on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. TrxR1 Polyclonal Antibody (Product # PA5-34685) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TrxR1 Polyclonal Antibody detects TrxR1 protein at cytoplasm on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. TrxR1 Polyclonal Antibody (Product # PA5-34685) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis showing protein expression of TXNRD1, HSPA4L and ATP1B1 in ( A ) the fresh pre-freeze (PF) and ( B ) frozen-thawed (FT) sperm from boars of the good and poor semen freezability (GSF and PSF, respectively) groups.
