sc-17787

antibody from Santa Cruz Biotechnology

Targeting: TUBG1 TUBG, TUBGCP1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-17787

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-17787, RRID:AB_628417

Product name

Anti-TUBG1

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

References

Submitted references

Angelman syndrome protein UBE3A interacts with primary microcephaly protein ASPM, localizes to centrosomes and regulates chromosome segregation.

Singhmar P, Kumar A
PloS one 2011;6(5):e20397

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A novel class of cyclin-dependent kinase inhibitors identified by molecular docking act through a unique mechanism.

Corsino P, Horenstein N, Ostrov D, Rowe T, Law M, Barrett A, Aslanidi G, Cress WD, Law B
The Journal of biological chemistry 2009 Oct 23;284(43):29945-55

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band corresponding to the predicted size in kDa (+/-20%).

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

A-431

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 51

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.