Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [8]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX113286 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113286, RRID:AB_1952442
- Product name
- gamma Tubulin antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Zebrafish
- Host
- Rabbit
Submitted references Cellular responses to ionizing radiation change quickly over time during early development in zebrafish.
Skin Delivery of Clec4a Small Hairpin RNA Elicited an Effective Antitumor Response by Enhancing CD8(+) Immunity In Vivo.
Efficient Multiple Genome Modifications Induced by the crRNAs, tracrRNA and Cas9 Protein Complex in Zebrafish.
The UBC Domain Is Required for BRUCE to Promote BRIT1/MCPH1 Function in DSB Signaling and Repair Post Formation of BRUCE-USP8-BRIT1 Complex.
Fat-Dachsous signaling coordinates cartilage differentiation and polarity during craniofacial development.
MJ-66 induces malignant glioma cells G2/M phase arrest and mitotic catastrophe through regulation of cyclin B1/Cdk1 complex.
Honjo Y, Ichinohe T
Cell biology international 2019 May;43(5):516-527
Cell biology international 2019 May;43(5):516-527
Skin Delivery of Clec4a Small Hairpin RNA Elicited an Effective Antitumor Response by Enhancing CD8(+) Immunity In Vivo.
Weng TY, Li CJ, Li CY, Hung YH, Yen MC, Chang YW, Chen YH, Chen YL, Hsu HP, Chang JY, Lai MD
Molecular therapy. Nucleic acids 2017 Dec 15;9:419-427
Molecular therapy. Nucleic acids 2017 Dec 15;9:419-427
Efficient Multiple Genome Modifications Induced by the crRNAs, tracrRNA and Cas9 Protein Complex in Zebrafish.
Kotani H, Taimatsu K, Ohga R, Ota S, Kawahara A
PloS one 2015;10(5):e0128319
PloS one 2015;10(5):e0128319
The UBC Domain Is Required for BRUCE to Promote BRIT1/MCPH1 Function in DSB Signaling and Repair Post Formation of BRUCE-USP8-BRIT1 Complex.
Ge C, Che L, Du C
PloS one 2015;10(12):e0144957
PloS one 2015;10(12):e0144957
Fat-Dachsous signaling coordinates cartilage differentiation and polarity during craniofacial development.
Le Pabic P, Ng C, Schilling TF
PLoS genetics 2014 Oct;10(10):e1004726
PLoS genetics 2014 Oct;10(10):e1004726
MJ-66 induces malignant glioma cells G2/M phase arrest and mitotic catastrophe through regulation of cyclin B1/Cdk1 complex.
Liu WT, Chen C, Lu IC, Kuo SC, Lee KH, Chen TL, Song TS, Lu YL, Gean PW, Hour MJ
Neuropharmacology 2014 Nov;86:219-27
Neuropharmacology 2014 Nov;86:219-27
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE GTX113286 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Molt-4 (GTX27912) 10% SDS PAGE GTX113286 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- gamma Tubulin antibody detects gamma Tubulin protein by western blot analysis.A. 50 ?g rat kidney whole lysate/extract 10 % SDS-PAGEgamma Tubulin antibody (GTX113286) dilution: 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- gamma Tubulin antibody detects gamma Tubulin protein by western blot analysis.A. 30 ?g Jurkat whole cell lysate/extract B. 30 ?g Raji whole cell lysate/extract 10 % SDS-PAGEgamma Tubulin antibody (GTX113286) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- gamma Tubulin antibody detects gamma Tubulin protein by western blot analysis. NIH3T3 whole cell extracts (30 μg) and rat tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody (GTX113286) diluted by 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- gamma Tubulin antibody detects gamma Tubulin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody (GTX113286) diluted by 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- gamma Tubulin antibody detects gamma Tubulin protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody (GTX113286) diluted at a dilution of 1:1000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- gamma Tubulin antibody detects gamma Tubulin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody (GTX113286) diluted at a dilution of 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed 293T, using gamma Tubulin(GTX113286) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed 293T, using gamma Tubulin(GTX113286) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of gamma Tubulin protein from Jurkat whole cell extracts using 5 £gg of gamma Tubulin antibody (GTX113286).Western blot analysis was performed using gamma Tubulin antibody (GTX113286).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- gamma Tubulin antibody detects gamma Tubulin protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. gamma Tubulin antibody (GTX113286) dilution: 1:500.
