Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX15794 - Provider product page
- Provider
- GeneTex
- Product name
- gamma Tubulin antibody [4D11]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Western blot analysis of gamma Tubulin in 50μg of various whole cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with gamma Tubulin antibody [4D11] at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a proper secondary antibody. Membranes were washed and chemiluminescent detection was performed.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of gamma Tubulin (green) in (A) HeLa and (B) U2-OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with gamma Tubulin antibody [4D11] at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with a proper secondary antibody. F-Actin (red) was stained with phalloidin, nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on normal deparaffinized human colon tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with or without gamma Tubulin antibody [4D11] overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjμgated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.