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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- NB100-74421 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-74421, RRID:AB_1050000
- Product name
- Mouse Monoclonal gamma Tubulin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. gamma Tubulin (4D11)
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100ug
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: gamma Tubulin Antibody (4D11) [NB100-74421] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing g-Tubulin at a dilution of 1:1000 overnight at 4C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: gamma Tubulin Antibody (4D11) [NB100-74421] - Both normal and cancer biopsies of deparaffinized human Breast carcinoma tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: gamma Tubulin Antibody (4D11) [NB100-74421] - Both normal and cancer biopsies of deparaffinized human Colon tissue.
