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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- MA5-24588 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SIX1 Monoclonal Antibody (CL0185)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: CFKEKSRGVL REWYAHNPYP SPREKRELAE ATGLTTTQVS NWFKNRRQRD RAAEAKEREN TENNNSSSNK QNQLSPLEGG KPLMSSSEEE FSPPQSPDQN SVLLLQGNMG HARSSNYSLP GLTASQPSHG LQTHQHQLQD S Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 99%.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CL0185
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SIX1 in Lane1: Marker (kDa), Lane 2: Human cell line RH-30, Lane 3: Human cell line CACO-2. Samples were probed using a SIX1 Monoclonal Antibody (Product # MA5-24588).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SIX1 in Lane1: Marker (kDa), Lane 2: Human cell line RH-30, Lane 3: Human cell line CACO-2. Samples were probed using a SIX1 Monoclonal Antibody (Product # MA5-24588).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from RH-30 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using SIX1 Monoclonal Antibody (CL0185) (Product # MA5-24588). Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of SIX1 in RH30 cell line using SIX1 Monoclonal Antibody (Product # MA5-24588), showing spotty nuclear staining in green. Microtubule probes are visualized in red (where available).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SIX1 in human liver tissue shows absence of immunoreactivity (negative control). Samples were probed using a SIX1 Monoclonal Antibody (Product # MA5-24588).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SIX1 in human striated muscle shows strong nuclear immunoreactivity in the myocytes. Samples were probed using a SIX1 Monoclonal Antibody (Product # MA5-24588).
