PAB27989

antibody from Abnova Corporation

Targeting: APOOL AAIR8193, CXorf33, FAM121A, Mic27, MICOS27, UNQ8193

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB27989

Provider

Abnova Corporation

Product name

APOOL polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant APOOL

Antigen sequence

ATLGATVCYPVQSVIIAKVTAKKVYATSQQIFGAV
KSLWTKSSKEESLPKPKEKTKLGSSSEIEVPAKTT
HVLKHSVPLPTELSSEAKTKSESTSGATQFMPDPK
LMDHGQSHPED


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of cell lysates with APOOL polyclonal antibody ( Cat # PAB27989 ) at 1:100-1:500 dilution.Lane 1: NIH-3T3Lane 2: NBT-II

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4 Lane 2: EFO-21 Lane 3: A-431 Lane 4: Liver Lane 5: Tonsil with APOOL polyclonal antibody ( Cat # PAB27989 ) at 1:250 - 1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with APOOL polyclonal antibody ( Cat # PAB27989 ) at 1-4 ug/ml dilution shows positivity in mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach with APOOL polyclonal antibody ( Cat # PAB27989 ) shows strong cytoplasmic positivity in glandular cells at 1:500 - 1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)