LS-B7259

antibody from LSBio

Targeting: VDR NR1I1, PPP1R163

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-B7259

Provider

LSBio

Product name

IHC-plus™ Vitamin D Receptor / VDR Antibody (aa181-230) LS-B7259

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of Anti-VDR antibody (LS-B7259, 1:1000 dilution; 15 µg of lysate per lane). Lane 1: A431 cell line. Lane 2: MCF-7 cell line. Lane 3: MCF-7 cell line blocked with synthesized peptide. Antibody produced band at ~48 kDa (Isoform 1) but did not produce band when blocked.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-VDR antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-VDR antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.