sc-13133

antibody from Santa Cruz Biotechnology

Targeting: VDR NR1I1, PPP1R163

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-13133

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-13133, RRID:AB_628040

Product name

Anti-VDR

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

References

Submitted references

A novel bile acid-activated vitamin D receptor signaling in human hepatocytes.

Han S, Li T, Ellis E, Strom S, Chiang JY
Molecular endocrinology (Baltimore, Md.) 2010 Jun;24(6):1151-64

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Effects of supplemental vitamin D and calcium on oxidative DNA damage marker in normal colorectal mucosa: a randomized clinical trial.

Fedirko V, Bostick RM, Long Q, Flanders WD, McCullough ML, Sidelnikov E, Daniel CR, Rutherford RE, Shaukat A
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 2010 Jan;19(1):280-91

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Cholecalciferol supplementation alters calcitriol-responsive monocyte proteins and decreases inflammatory cytokines in ESRD.

Stubbs JR, Idiculla A, Slusser J, Menard R, Quarles LD
Journal of the American Society of Nephrology : JASN 2010 Feb;21(2):353-61

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Parathyroid Klotho and FGF-receptor 1 expression decline with renal function in hyperparathyroid patients with chronic kidney disease and kidney transplant recipients

Tijana Krajisnik, Hannes Olauson, Majd A I Mirza, Per Hellman, Göran Åkerström, Gunnar Westin, Tobias E Larsson, Peyman Björklund
Kidney International 2010 Aug;78(10):1024-1032

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Multi-step immunofluorescent analysis of vitamin D receptor loci and myosin heavy chain isoforms in human skeletal muscle.

Ceglia L, da Silva Morais M, Park LK, Morris E, Harris SS, Bischoff-Ferrari HA, Fielding RA, Dawson-Hughes B
Journal of molecular histology 2010 Apr;41(2-3):137-42

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Control of TCF-4 expression by VDR and vitamin D in the mouse mammary gland and colorectal cancer cell lines.

Beildeck ME, Islam M, Shah S, Welsh J, Byers SW
PloS one 2009 Nov 17;4(11):e7872

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20-Hydroxyvitamin D3, a product of vitamin D3 hydroxylation by cytochrome P450scc, stimulates keratinocyte differentiation.

Zbytek B, Janjetovic Z, Tuckey RC, Zmijewski MA, Sweatman TW, Jones E, Nguyen MN, Slominski AT
The Journal of investigative dermatology 2008 Sep;128(9):2271-80

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Functional vitamin D receptor (VDR) in the t-tubules of cardiac myocytes: VDR knockout cardiomyocyte contractility.

Tishkoff DX, Nibbelink KA, Holmberg KH, Dandu L, Simpson RU
Endocrinology 2008 Feb;149(2):558-64

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 48

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.