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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- MA5-14617 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VDR Monoclonal Antibody (9A7gamma.E10.E4)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA5-14617 targets Vitamin D Receptor in GS and WB applications and shows reactivity with Amphibian, Chicken, Fish, Hamster, Human, mouse, Porcine, and Rat samples.
- Antibody clone number
- 9A7gamma.E10.E4
- Concentration
- 0.2 mg/mL
Submitted references Vitamin D and androgen receptor-targeted therapy for triple-negative breast cancer.
Mitochondrial translocation of vitamin D receptor is mediated by the permeability transition pore in human keratinocyte cell line.
Crosstalk between the peroxisome proliferator-activated receptor γ (PPARγ) and the vitamin D receptor (VDR) in human breast cancer cells: PPARγ binds to VDR and inhibits 1α,25-dihydroxyvitamin D3 mediated transactivation.
Mammary adipocytes bioactivate 25-hydroxyvitamin D₃ and signal via vitamin D₃ receptor, modulating mammary epithelial cell growth.
Functionality of unliganded VDR in breast cancer cells: repressive action on CYP24 basal transcription.
Influence of different calcium supplies and a single vitamin D injection on vitamin D receptor and calbindin D9k immunoreactivities in the gastrointestinal tract of goat kids.
Chemotherapeutic effect of calcidiol derivative B3CD in a neuroblastoma xenograft model.
1,25 Dihydroxyvitamin D(3) receptor expression in superficial transitional cell carcinoma of the bladder: a possible prognostic factor?
Induction of apoptosis by 1,25-dihydroxyvitamin D3 in MCF-7 Vitamin D3-resistant variant can be sensitized by TPA.
Induction of apoptosis by 1,25-dihydroxyvitamin D3 in MCF-7 Vitamin D3-resistant variant can be sensitized by TPA.
Characterization of mammary tumor cell lines from wild type and vitamin D3 receptor knockout mice.
Characterization of mammary tumor cell lines from wild type and vitamin D3 receptor knockout mice.
Thakkar A, Wang B, Picon-Ruiz M, Buchwald P, Ince TA
Breast cancer research and treatment 2016 May;157(1):77-90
Breast cancer research and treatment 2016 May;157(1):77-90
Mitochondrial translocation of vitamin D receptor is mediated by the permeability transition pore in human keratinocyte cell line.
Silvagno F, Consiglio M, Foglizzo V, Destefanis M, Pescarmona G
PloS one 2013;8(1):e54716
PloS one 2013;8(1):e54716
Crosstalk between the peroxisome proliferator-activated receptor γ (PPARγ) and the vitamin D receptor (VDR) in human breast cancer cells: PPARγ binds to VDR and inhibits 1α,25-dihydroxyvitamin D3 mediated transactivation.
Alimirah F, Peng X, Yuan L, Mehta RR, von Knethen A, Choubey D, Mehta RG
Experimental cell research 2012 Nov 15;318(19):2490-7
Experimental cell research 2012 Nov 15;318(19):2490-7
Mammary adipocytes bioactivate 25-hydroxyvitamin D₃ and signal via vitamin D₃ receptor, modulating mammary epithelial cell growth.
Ching S, Kashinkunti S, Niehaus MD, Zinser GM
Journal of cellular biochemistry 2011 Nov;112(11):3393-405
Journal of cellular biochemistry 2011 Nov;112(11):3393-405
Functionality of unliganded VDR in breast cancer cells: repressive action on CYP24 basal transcription.
Alimirah F, Vaishnav A, McCormick M, Echchgadda I, Chatterjee B, Mehta RG, Peng X
Molecular and cellular biochemistry 2010 Sep;342(1-2):143-50
Molecular and cellular biochemistry 2010 Sep;342(1-2):143-50
Influence of different calcium supplies and a single vitamin D injection on vitamin D receptor and calbindin D9k immunoreactivities in the gastrointestinal tract of goat kids.
Sidler-Lauff K, Boos A, Kraenzlin M, Liesegang A
Journal of animal science 2010 Nov;88(11):3598-610
Journal of animal science 2010 Nov;88(11):3598-610
Chemotherapeutic effect of calcidiol derivative B3CD in a neuroblastoma xenograft model.
Lange TS, Zou Y, Singh RK, Kim KK, Kristjansdottir K, Sholler GL, Brard L
Chemical biology & drug design 2010 Aug;76(2):164-73
Chemical biology & drug design 2010 Aug;76(2):164-73
1,25 Dihydroxyvitamin D(3) receptor expression in superficial transitional cell carcinoma of the bladder: a possible prognostic factor?
Sahin MO, Canda AE, Yorukoglu K, Mungan MU, Sade M, Kirkali Z
European urology 2005 Jan;47(1):52-7
European urology 2005 Jan;47(1):52-7
Induction of apoptosis by 1,25-dihydroxyvitamin D3 in MCF-7 Vitamin D3-resistant variant can be sensitized by TPA.
Narvaez CJ, Byrne BM, Romu S, Valrance M, Welsh J
The Journal of steroid biochemistry and molecular biology 2003 Feb;84(2-3):199-209
The Journal of steroid biochemistry and molecular biology 2003 Feb;84(2-3):199-209
Induction of apoptosis by 1,25-dihydroxyvitamin D3 in MCF-7 Vitamin D3-resistant variant can be sensitized by TPA.
Narvaez CJ, Byrne BM, Romu S, Valrance M, Welsh J
The Journal of steroid biochemistry and molecular biology 2003 Feb;84(2-3):199-209
The Journal of steroid biochemistry and molecular biology 2003 Feb;84(2-3):199-209
Characterization of mammary tumor cell lines from wild type and vitamin D3 receptor knockout mice.
Zinser GM, McEleney K, Welsh J
Molecular and cellular endocrinology 2003 Feb 28;200(1-2):67-80
Molecular and cellular endocrinology 2003 Feb 28;200(1-2):67-80
Characterization of mammary tumor cell lines from wild type and vitamin D3 receptor knockout mice.
Zinser GM, McEleney K, Welsh J
Molecular and cellular endocrinology 2003 Feb 28;200(1-2):67-80
Molecular and cellular endocrinology 2003 Feb 28;200(1-2):67-80
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of VDR was performed using 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VDR (9A7gamma.E10.E4) Rat Monoclonal Antibody (Product # MA5-14617) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rat IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A-11006) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Evaluation of androgen and vitamin D receptor agonists response in BC lines: a Western blot analysis of 15 breast cancer cell lines for ER, AR, VDR, PR, and Her2 expression. Two AR+ and two AR- prostate cancer cell lines, LNCaP, LAPC-4, PC-3, and DU-145, respectively, were used as controls for AR expression. 30 ug extract was loaded in each lane of a 4-12 % 20-well SDS-PAGE gel, which was transferred onto a nitrocellulose membrane and probed with the following antibodies: ERalpha (1:750, Santa Cruz sc-8002), progesterone receptor (1:1000, Thermofisher Scientific MA1-410), HER2 (1:2000, Abcam ab2428), androgen receptor (N-20) (1:1000, Abcam sc-816), vitamin D receptor (1:1000, Thermofisher Scientific MA5-14617). beta-Actin is used as a loading control (1:5000, Sigma A2228). The results of VDR and AR agonist treatment experiments in panels b and c are plotted as percent viability of the cells in comparison to the vehicle control. Error bars represent mean +- standard error of mean (SEM). These experiments were repeated at least three times. The statistical significance of the drug treatments was determined using two-tailed Student's T -test. * P < 0.05, ** P < 0.01. Cal calcitriol, DHT dihydrotestosterone. b VDR agonist calcitriol treatment on cancer cell lines: breast cancer cell lines [ black VDR(+) and hatched VDR(- or low)] were plated in their respective media with complete serum in triplicates. 24-48 h after plating, the cells were treated with 100 nM calcitriol fo
