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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-5285 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-5285, RRID:AB_628447
- Product name
- Anti-WEE1
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references Prediction of Associations between microRNAs and Gene Expression in Glioma Biology.
Human immunodeficiency virus type 1 Vpr binds to the N lobe of the Wee1 kinase domain and enhances kinase activity for CDC2.
Depletion of Wee-1 kinase is necessary for both human immunodeficiency virus type 1 Vpr- and gamma irradiation-induced apoptosis.
Wuchty S, Arjona D, Li A, Kotliarov Y, Walling J, Ahn S, Zhang A, Maric D, Anolik R, Zenklusen JC, Fine HA
PloS one 2011 Feb 16;6(2):e14681
PloS one 2011 Feb 16;6(2):e14681
Human immunodeficiency virus type 1 Vpr binds to the N lobe of the Wee1 kinase domain and enhances kinase activity for CDC2.
Kamata M, Watanabe N, Nagaoka Y, Chen IS
Journal of virology 2008 Jun;82(12):5672-82
Journal of virology 2008 Jun;82(12):5672-82
Depletion of Wee-1 kinase is necessary for both human immunodeficiency virus type 1 Vpr- and gamma irradiation-induced apoptosis.
Yuan H, Xie YM, Chen IS
Journal of virology 2003 Feb;77(3):2063-70
Journal of virology 2003 Feb;77(3):2063-70
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- No bands detected.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 72
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human placenta shows strong cytoplasmic and nuclear positivity in cytotrophoblasts.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
