Antibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
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- Product number
- MA1-25492 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 14-3-3 beta/zeta Monoclonal Antibody (22-IID8B)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Recommended positive controls: HeLa cell lysate.
- Antibody clone number
- 22-IID8B
- Concentration
- 1 mg/mL
Submitted references A combined proteome and ultrastructural localization analysis of 14-3-3 proteins in transformed human amnion (AMA) cells: definition of a framework to study isoform-specific differences.
Moreira JM, Shen T, Ohlsson G, Gromov P, Gromova I, Celis JE
Molecular & cellular proteomics : MCP 2008 Jul;7(7):1225-40
Molecular & cellular proteomics : MCP 2008 Jul;7(7):1225-40
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human epidermoid carcinoma A431 cell lysate, probed with 14-3-3 beta/zeta Monoclonal Antibody (22-IID8B) (Product # MA1-25492).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of 14-3-3 protein beta/zeta was achieved by transfecting MCF7 with 14-3-3 protein beta/zeta specific siRNAs (Silencer® select Product # s14961, s14962, s14972). Western blot analysis (Fig. a) was performed using Membrane enriched extracts from the 14-3-3 protein beta/zeta knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with 14-3-3 beta/zeta Monoclonal Antibody (22-IID8B) (Product # MA1-25492, 1:1000 dilution ) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:10,000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to 14-3-3 protein beta/zeta.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-14-3-3 beta/zeta Monoclonal Antibody (22-IID8B) (Product # MA1-25492) and a 28 kDa band corresponding to 14-3-3 protein beta/zeta was observed across the cell lines and tissues tested. Membrane enriched extracts (30 µg lysate) of MCF7 (Lane 1), HeLa (Lane 2), PC-3 (Lane 3), A549 (Lane 4), Mouse Skeletal Muscle (Lane 5), Rat Skeletal Muscle (Lane 6), Mouse Brain (Lane 7), Rat Brain (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:10,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).