GTX32279

antibody from GeneTex

Targeting: YWHAZ 14-3-3-zeta, KCIP-1, YWHAD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX32279

Provider

GeneTex

Product name

14-3-3 zeta (phospho Ser58) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Storage

Shipped at 4 ¢XC. Upon delivery aliquot and store at -20 ¢XC for one year. Avoid freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of 14-3-3 zeta (pS58) expression in MCF7 (A); A549 (B); SP2/0 (C); rat liver (D); NIH3T3 (E); A431 (F) whole cell lysates.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of 14-3-3 zeta (pS58) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of 14-3-3 zeta (pS58) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.