687802

antibody from BioLegend

Targeting: YWHAZ 14-3-3-zeta, KCIP-1, YWHAD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

687802

Provider

BioLegend

Proper citation

BioLegend Cat#687802, RRID:AB_2617006

Product name

Purified anti-14-3-3 ζ/δ

Antibody type

Monoclonal

Antigen

Full length human 14-3-3 ζ/δ recombinant protein expressed in E. coli.

Reactivity

Human, Mouse, Rat

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W15121A

Vial size

100 µg

Concentration

0.5 μ/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

HeLa cells were fixed with methanol for five minutes and blocked with 1% BSA for 60 minutes. Then the cells were intracellularly stained with 2.0 µg/ml purified anti-14-3-3 ζ/δ (clone W15121A) antibody for one hour, followed by staining with Alexa Fluor® 594 conjugated anti-rat IgG antibody (red) and DAPI (blue) for one hour at room temperature. The image was captured with a 60X objective.

Conjugate

Unconjugated