Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 687802 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#687802, RRID:AB_2617006
- Product name
- Purified anti-14-3-3 ζ/δ
- Antibody type
- Monoclonal
- Antigen
- Full length human 14-3-3 ζ/δ recombinant protein expressed in E. coli.
- Reactivity
- Human, Mouse, Rat
- Host
- Rat
- Conjugate
- Unconjugated
- Antibody clone number
- W15121A
- Vial size
- 100 µg
- Concentration
- 0.5 μ/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- HeLa cells were fixed with methanol for five minutes and blocked with 1% BSA for 60 minutes. Then the cells were intracellularly stained with 2.0 µg/ml purified anti-14-3-3 ζ/δ (clone W15121A) antibody for one hour, followed by staining with Alexa Fluor® 594 conjugated anti-rat IgG antibody (red) and DAPI (blue) for one hour at room temperature. The image was captured with a 60X objective.
- Conjugate
- Unconjugated