500-P109-1MG

antibody from Invitrogen Antibodies

Targeting: CCL21 6Ckine, CKb9, ECL, exodus-2, SCYA21, SLC, TCA4

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P109-1MG

Provider

Invitrogen Antibodies

Product name

CCL21 Polyclonal Antibody, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: discontinued. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Exodus-2 (CCL21). Anti-Human Exodus-2 (CCL21)-specific antibody was purified by affinity chromatography employing an immobilized Human Exodus-2 (CCL21) matrix. Sandwich ELISA: To detect Human Exodus-2 (CCL21) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Exodus-2 (CCL21) (500-P109Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Exodus-2 (CCL21). Western Blot: To detect Human Exodus-2 (CCL21) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Exodus-2 (CCL21) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Reactivity

Human

Host

Rabbit

Vial size

1 mg

Concentration

0.1-1.0 mg/mL

Storage

-20°C

References

Submitted references

Chemokine axes in breast cancer: factors of the tumor microenvironment reshape the CCR7-driven metastatic spread of luminal-A breast tumors.

Weitzenfeld P, Kossover O, Körner C, Meshel T, Wiemann S, Seliktar D, Legler DF, Ben-Baruch A
Journal of leukocyte biology 2016 Jun;99(6):1009-25

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The three-dimensional structure of human splenic white pulp compartments.

Steiniger B, Rüttinger L, Barth PJ
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2003 May;51(5):655-64

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Isolation and characterization of dermal lymphatic and blood endothelial cells reveal stable and functionally specialized cell lineages.

Kriehuber E, Breiteneder-Geleff S, Groeger M, Soleiman A, Schoppmann SF, Stingl G, Kerjaschki D, Maurer D
The Journal of experimental medicine 2001 Sep 17;194(6):797-808

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect Human Exodus-2 (CCL21) by Western Blot analysis CCL21 Polyclonal Antibody (Product # 500-P109-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Exodus-2 (CCL21) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect Human Exodus-2 (CCL21) by Western Blot analysis CCL21 Polyclonal Antibody (Product # 500-P109-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Exodus-2 (CCL21) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect Human Exodus-2 (CCL21) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CCL21 Polyclonal Antibody (Product # 500-P109-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CCL21 Polyclonal Antibody, Biotin (Product # 500-P109BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Exodus-2 (CCL21).