Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- sc-5579 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-5579, RRID:AB_633731
- Product name
- Anti-AHR
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Aryl hydrocarbon receptor facilitates DNA strand breaks and 8-oxo-2'-deoxyguanosine formation by the aldo-keto reductase product benzo[a]pyrene-7,8-dione.
Concordant regulation of translation and mRNA abundance for hundreds of targets of a human microRNA.
Characterization of the pattern of the nongenomic signaling pathway through which TCDD-induces early inflammatory responses in U937 human macrophages.
Characterization of MCF mammary epithelial cells overexpressing the Arylhydrocarbon receptor (AhR).
Targeting of aryl hydrocarbon receptor-mediated activation of cyclooxygenase-2 expression by the indole-3-carbinol metabolite 3,3'-diindolylmethane in breast cancer cells.
Aryl hydrocarbon receptor pathway activation enhances gastric cancer cell invasiveness likely through a c-Jun-dependent induction of matrix metalloproteinase-9.
Potential therapeutic significance of increased expression of aryl hydrocarbon receptor in human gastric cancer.
Park JH, Mangal D, Frey AJ, Harvey RG, Blair IA, Penning TM
The Journal of biological chemistry 2009 Oct 23;284(43):29725-34
The Journal of biological chemistry 2009 Oct 23;284(43):29725-34
Concordant regulation of translation and mRNA abundance for hundreds of targets of a human microRNA.
Hendrickson DG, Hogan DJ, McCullough HL, Myers JW, Herschlag D, Ferrell JE, Brown PO
PLoS biology 2009 Nov;7(11):e1000238
PLoS biology 2009 Nov;7(11):e1000238
Characterization of the pattern of the nongenomic signaling pathway through which TCDD-induces early inflammatory responses in U937 human macrophages.
Sciullo EM, Dong B, Vogel CF, Matsumura F
Chemosphere 2009 Mar;74(11):1531-7
Chemosphere 2009 Mar;74(11):1531-7
Characterization of MCF mammary epithelial cells overexpressing the Arylhydrocarbon receptor (AhR).
Wong PS, Li W, Vogel CF, Matsumura F
BMC cancer 2009 Jul 15;9:234
BMC cancer 2009 Jul 15;9:234
Targeting of aryl hydrocarbon receptor-mediated activation of cyclooxygenase-2 expression by the indole-3-carbinol metabolite 3,3'-diindolylmethane in breast cancer cells.
Degner SC, Papoutsis AJ, Selmin O, Romagnolo DF
The Journal of nutrition 2009 Jan;139(1):26-32
The Journal of nutrition 2009 Jan;139(1):26-32
Aryl hydrocarbon receptor pathway activation enhances gastric cancer cell invasiveness likely through a c-Jun-dependent induction of matrix metalloproteinase-9.
Peng TL, Chen J, Mao W, Song X, Chen MH
BMC cell biology 2009 Apr 16;10:27
BMC cell biology 2009 Apr 16;10:27
Potential therapeutic significance of increased expression of aryl hydrocarbon receptor in human gastric cancer.
Peng TL, Chen J, Mao W, Liu X, Tao Y, Chen LZ, Chen MH
World journal of gastroenterology 2009 Apr 14;15(14):1719-29
World journal of gastroenterology 2009 Apr 14;15(14):1719-29
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 96
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human lymph node shows cytoplasmic positivity in reaction center cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
