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LearnH00000196-M02
antibody from Novus Biologicals
Targeting: AHR
bHLHe76
Western blot ELISA Immunocytochemistry Immunoprecipitation
Blocking/Neutralizing Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00000196-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000196-M02, RRID:AB_668776
- Product name
- Mouse Monoclonal AHR Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. aryl hydrocarbon receptor
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Protective effects of coffee against oxidative stress induced by the tobacco carcinogen benzo[α]pyrene.
Cross-talk between aryl hydrocarbon receptor and the inflammatory response: a role for nuclear factor-κB.
Gender matters: estrogen receptor alpha (ERα) and histone deacetylase (HDAC) 1 and 2 control the gender-specific transcriptional regulation of human uridine diphosphate glucuronosyltransferases genes (UGT1A).
Malassezia-derived indoles activate the aryl hydrocarbon receptor and inhibit Toll-like receptor-induced maturation in monocyte-derived dendritic cells.
Diminished carcinogen detoxification is a novel mechanism for hypoxia-inducible factor 1-mediated genetic instability.
Kalthoff S, Landerer S, Reich J, Strassburg CP
Free radical biology & medicine 2017 Jul;108:66-76
Free radical biology & medicine 2017 Jul;108:66-76
Cross-talk between aryl hydrocarbon receptor and the inflammatory response: a role for nuclear factor-κB.
Vogel CF, Khan EM, Leung PS, Gershwin ME, Chang WL, Wu D, Haarmann-Stemmann T, Hoffmann A, Denison MS
The Journal of biological chemistry 2014 Jan 17;289(3):1866-75
The Journal of biological chemistry 2014 Jan 17;289(3):1866-75
Gender matters: estrogen receptor alpha (ERα) and histone deacetylase (HDAC) 1 and 2 control the gender-specific transcriptional regulation of human uridine diphosphate glucuronosyltransferases genes (UGT1A).
Kalthoff S, Winkler A, Freiberg N, Manns MP, Strassburg CP
Journal of hepatology 2013 Oct;59(4):797-804
Journal of hepatology 2013 Oct;59(4):797-804
Malassezia-derived indoles activate the aryl hydrocarbon receptor and inhibit Toll-like receptor-induced maturation in monocyte-derived dendritic cells.
Vlachos C, Schulte BM, Magiatis P, Adema GJ, Gaitanis G
The British journal of dermatology 2012 Sep;167(3):496-505
The British journal of dermatology 2012 Sep;167(3):496-505
Diminished carcinogen detoxification is a novel mechanism for hypoxia-inducible factor 1-mediated genetic instability.
Schults MA, Timmermans L, Godschalk RW, Theys J, Wouters BG, van Schooten FJ, Chiu RK
The Journal of biological chemistry 2010 May 7;285(19):14558-64
The Journal of biological chemistry 2010 May 7;285(19):14558-64
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR over-expressed 293 cell line, cotransfected with AHR Validated Chimera RNAi ( Cat # H00000196-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AHR monoclonal antibody (M02), clone 3B12 (Cat # H00000196-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR expression in transfected 293T cell line by AHR monoclonal antibody (M02), clone 3B12.Lane 1: AHR transfected lysate(96.147 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: AHR Antibody (3B12) [H00000196-M02] - Detection limit for recombinant GST tagged AHR is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: AHR Antibody (3B12) [H00000196-M02] - Analysis of monoclonal antibody to AHR on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: AHR Antibody (3B12) [H00000196-M02] - Analysis of AHR transfected lysate using anti-AHR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AHR MaxPab rabbit polyclonal antibody.
