Explore
Validate
LearnNB300-515
antibody from Novus Biologicals
Targeting: AHR
bHLHe76
Western blot
ELISA Immunocytochemistry Immunoprecipitation Immunohistochemistry Flow cytometry Gel shift Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB300-515 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-515, RRID:AB_10003031
- Product name
- Mouse Monoclonal AHR Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Detects the Aryl Hydrocarbon Receptor (AHR).
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 uL
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Expression of Serpin Peptidase Inhibitor B2 (SERPINB2) is regulated by Aryl hydrocarbon receptor (AhR).
Carbidopa is an activator of aryl hydrocarbon receptor with potential for cancer therapy.
Ikaros Inhibits Group 3 Innate Lymphoid Cell Development and Function by Suppressing the Aryl Hydrocarbon Receptor Pathway.
Expression of aryl hydrocarbon receptor (AHR) and AHR-interacting protein in pituitary adenomas: pathological and clinical implications.
Role of GAC63 in transcriptional activation mediated by the aryl hydrocarbon receptor.
7-ketocholesterol is an endogenous modulator for the arylhydrocarbon receptor.
Characterization of the AhR-hsp90-XAP2 core complex and the role of the immunophilin-related protein XAP2 in AhR stabilization.
Brauze D, Kiwerska K, Bednarek K, Grenman R, Janiszewska J, Giefing M, Jarmuz-Szymczak M
Chemico-biological interactions 2019 Aug 25;309:108700
Chemico-biological interactions 2019 Aug 25;309:108700
Carbidopa is an activator of aryl hydrocarbon receptor with potential for cancer therapy.
Ogura J, Miyauchi S, Shimono K, Yang S, Gonchigar S, Ganapathy V, Bhutia YD
The Biochemical journal 2017 Sep 28;474(20):3391-3402
The Biochemical journal 2017 Sep 28;474(20):3391-3402
Ikaros Inhibits Group 3 Innate Lymphoid Cell Development and Function by Suppressing the Aryl Hydrocarbon Receptor Pathway.
Li S, Heller JJ, Bostick JW, Lee A, Schjerven H, Kastner P, Chan S, Chen ZE, Zhou L
Immunity 2016 Jul 19;45(1):185-97
Immunity 2016 Jul 19;45(1):185-97
Expression of aryl hydrocarbon receptor (AHR) and AHR-interacting protein in pituitary adenomas: pathological and clinical implications.
Jaffrain-Rea ML, Angelini M, Gargano D, Tichomirowa MA, Daly AF, Vanbellinghen JF, D'Innocenzo E, Barlier A, Giangaspero F, Esposito V, Ventura L, Arcella A, Theodoropoulou M, Naves LA, Fajardo C, Zacharieva S, Rohmer V, Brue T, Gulino A, Cantore G, Alesse E, Beckers A
Endocrine-related cancer 2009 Sep;16(3):1029-43
Endocrine-related cancer 2009 Sep;16(3):1029-43
Role of GAC63 in transcriptional activation mediated by the aryl hydrocarbon receptor.
Chen YH, Beischlag TV, Kim JH, Perdew GH, Stallcup MR
The Journal of biological chemistry 2006 May 5;281(18):12242-7
The Journal of biological chemistry 2006 May 5;281(18):12242-7
7-ketocholesterol is an endogenous modulator for the arylhydrocarbon receptor.
Savouret JF, Antenos M, Quesne M, Xu J, Milgrom E, Casper RF
The Journal of biological chemistry 2001 Feb 2;276(5):3054-9
The Journal of biological chemistry 2001 Feb 2;276(5):3054-9
Characterization of the AhR-hsp90-XAP2 core complex and the role of the immunophilin-related protein XAP2 in AhR stabilization.
Meyer BK, Perdew GH
Biochemistry 1999 Jul 13;38(28):8907-17
Biochemistry 1999 Jul 13;38(28):8907-17
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AHR Antibody (RPT9) [NB300-515] - Analysis of 40 ug of HEK293 lysate overexpressing Aryl Hydrocarbon Receptor (right lane) or empty vector control (left lane) and 10ul of PageRuler Prestained Protein Ladder.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor was performed by loading 20 ug of HEK293 lysate overexpressing Aryl Hydrocarbon Receptor (right lane B) or empty vector control (left lane A) and 10ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor in NIH/3T3 cells compared to an isotype control (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor in PC-3 cells compared to an isotype control (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: AHR Antibody (RPT9) [NB300-515] - Analysis of Aryl Hydrocarbon Receptor in Hela cells compared to an isotype control (blue).
