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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- 67461-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Arfaptin-1 Monoclonal Antibody (2A10B3)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Aliquoting is unnecessary for -20°C storage.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2A10B3
- Vial size
- 150 µL
- Concentration
- 1000 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 67461-1-Ig (ARFIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67461-1-Ig (Arfaptin-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67461-1-Ig (Arfaptin-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 Jurkat cells were intracellularly stained with 0.2 µg Anti-Human Arfaptin-1 (67461-1-Ig, Clone:2A10B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 µg Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (black). Cells were fixed with 90% MeOH.
