PA5-80307

antibody from Invitrogen Antibodies

Targeting: ACTR3 ARP3

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-80307

Provider

Invitrogen Antibodies

Product name

Arp3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human ACTR3/Actin-related protein 3.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Lamin A-mediated nuclear lamina integrity is required for proper ciliogenesis.

Fan JR, You LR, Wang WJ, Huang WS, Chu CT, Chi YH, Chen HC
EMBO reports 2020 Oct 5;21(10):e49680

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Arp3 in Lane A: HeLa Whole Cell Lysate (30 µg), Lane B: Jurkat Whole Cell Lysate (30 µg), Lane C: A549 Whole Cell Lysate (30 µg), Lane D: K562 Whole Cell Lysate (30 µg). Samples were probed using a Arp3 Polyclonal Antibody (Product # PA5-80307) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:47 kDa. Observed band size:45 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Arp3 Polyclonal Antibody (Product # PA5-80307) at 1:500 dilution. Lane A: HeLa Whole Cell Lysate, Lane B: Jurkat Whole Cell Lysate, Lane C: A549 Whole Cell Lysate, Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 45 kDa. (We are unsure of the identity of these extra bands).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of Arp3 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Arp3 Polyclonal Antibody (Product # PA5-80307, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Arp3 Immunoprecipitation using: Lane A: 0.5 mg K562 Whole Cell Lysate, Lane B: 0.5 mg Hela Whole Cell Lysate 4 µL with Arp3 Polyclonal Antibody (Product # PA5-80307) and 15 µL of 50 % Protein G agarose. Primary antibody: Arp3 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDa.