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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- TA300020 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA300020, RRID:AB_2064632
- Product name
- BLK Rabbit Polyclonal Antibody
- Antibody type
- Polyclonal
- Description
- BLK Rabbit Polyclonal Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- BLK
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- NULL
Submitted references Mutations at the BLK locus linked to maturity onset diabetes of the young and beta-cell dysfunction.
Borowiec M, Liew CW, Thompson R, Boonyasrisawat W, Hu J, Mlynarski WM, El Khattabi I, Kim SH, Marselli L, Rich SS, Krolewski AS, Bonner-Weir S, Sharma A, Sale M, Mychaleckyj JC, Kulkarni RN, Doria A
Proceedings of the National Academy of Sciences of the United States of America 2009 Aug 25;106(34):14460-5
Proceedings of the National Academy of Sciences of the United States of America 2009 Aug 25;106(34):14460-5
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- BLK Antibody (G1) (Cat. #TA300020) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the BLK antibody detected the BLK protein (arrow).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-BLK Pab (Cat. #TA300020) in mouse kidney tissue lysate. BLK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-BLK Pab (Cat. #TA300020) transiently transfected HEK-293 cell line lysate (1ug/lane). BLK pab(arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Immunofluorescence of BLK protein level by using anti-BLK antibody in human (Top) and murine (Bottom) islets. Dilution: 1:50
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Immunofluorescence of BLK protein level by using anti-BLK antibody in a tissue array of normal human tissues: spleen, tonsil, hari follicle, pancreas, leydig cells, salivary gland. Dilution: 1:50
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
- Validation comment
- IHC
