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- Antibody Data
- Antigen structure
- References [12]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF2344 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Carbonic Anhydrase IX/CA9 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Carbonic Anhydrase IX/CA9 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) CA9 is observed, 5% cross-reactivity with recombinant mouse (rm) CA14 is observed, and less than 1% cross-reactivity with rhCA1, -2, -3, -8, -10, -13, rmCA4, and -12 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q8VHB5- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The interactome of metabolic enzyme carbonic anhydrase IX reveals novel roles in tumor cell migration and invadopodia/MMP14-mediated invasion.
CD44 Interacts with HIF-2α to Modulate the Hypoxic Phenotype of Perinecrotic and Perivascular Glioma Cells.
Ascorbate availability affects tumor implantation-take rate and increases tumor rejection in Gulo(-/-) mice.
Simultaneous targeted activation of Notch1 and Vhl-disruption in the kidney proximal epithelial tubular cells in mice.
Bone marrow-derived mesenchymal stem cells drive lymphangiogenesis.
Expression patterns and subcellular localization of carbonic anhydrases are developmentally regulated during tooth formation.
Characterization of the adverse effects of nicotine on placental development: in vivo and in vitro studies.
Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
Cooperation between Pik3ca and p53 mutations in mouse mammary tumor formation.
VHL loss actuates a HIF-independent senescence programme mediated by Rb and p400.
CO2 chemosensing in rat oesophagus.
Carbonic anhydrases and mucosal vanilloid receptors help mediate the hyperemic response to luminal CO2 in rat duodenum.
Swayampakula M, McDonald PC, Vallejo M, Coyaud E, Chafe SC, Westerback A, Venkateswaran G, Shankar J, Gao G, Laurent EMN, Lou Y, Bennewith KL, Supuran CT, Nabi IR, Raught B, Dedhar S
Oncogene 2017 Nov 9;36(45):6244-6261
Oncogene 2017 Nov 9;36(45):6244-6261
CD44 Interacts with HIF-2α to Modulate the Hypoxic Phenotype of Perinecrotic and Perivascular Glioma Cells.
Johansson E, Grassi ES, Pantazopoulou V, Tong B, Lindgren D, Berg TJ, Pietras EJ, Axelson H, Pietras A
Cell reports 2017 Aug 15;20(7):1641-1653
Cell reports 2017 Aug 15;20(7):1641-1653
Ascorbate availability affects tumor implantation-take rate and increases tumor rejection in Gulo(-/-) mice.
Campbell EJ, Vissers MC, Dachs GU
Hypoxia (Auckland, N.Z.) 2016;4:41-52
Hypoxia (Auckland, N.Z.) 2016;4:41-52
Simultaneous targeted activation of Notch1 and Vhl-disruption in the kidney proximal epithelial tubular cells in mice.
Johansson E, Rönö B, Johansson M, Lindgren D, Möller C, Axelson H, Smith EM
Scientific reports 2016 Aug 5;6:30739
Scientific reports 2016 Aug 5;6:30739
Bone marrow-derived mesenchymal stem cells drive lymphangiogenesis.
Maertens L, Erpicum C, Detry B, Blacher S, Lenoir B, Carnet O, Péqueux C, Cataldo D, Lecomte J, Paupert J, Noel A
PloS one 2014;9(9):e106976
PloS one 2014;9(9):e106976
Expression patterns and subcellular localization of carbonic anhydrases are developmentally regulated during tooth formation.
Reibring CG, El Shahawy M, Hallberg K, Kannius-Janson M, Nilsson J, Parkkila S, Sly WS, Waheed A, Linde A, Gritli-Linde A
PloS one 2014;9(5):e96007
PloS one 2014;9(5):e96007
Characterization of the adverse effects of nicotine on placental development: in vivo and in vitro studies.
Holloway AC, Salomon A, Soares MJ, Garnier V, Raha S, Sergent F, Nicholson CJ, Feige JJ, Benharouga M, Alfaidy N
American journal of physiology. Endocrinology and metabolism 2014 Feb 15;306(4):E443-56
American journal of physiology. Endocrinology and metabolism 2014 Feb 15;306(4):E443-56
Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
Nature 2011 Jul 13;475(7355):226-30
Nature 2011 Jul 13;475(7355):226-30
Cooperation between Pik3ca and p53 mutations in mouse mammary tumor formation.
Adams JR, Xu K, Liu JC, Agamez NM, Loch AJ, Wong RG, Wang W, Wright KL, Lane TF, Zacksenhaus E, Egan SE
Cancer research 2011 Apr 1;71(7):2706-17
Cancer research 2011 Apr 1;71(7):2706-17
VHL loss actuates a HIF-independent senescence programme mediated by Rb and p400.
Young AP, Schlisio S, Minamishima YA, Zhang Q, Li L, Grisanzio C, Signoretti S, Kaelin WG Jr
Nature cell biology 2008 Mar;10(3):361-9
Nature cell biology 2008 Mar;10(3):361-9
CO2 chemosensing in rat oesophagus.
Akiba Y, Mizumori M, Kuo M, Ham M, Guth PH, Engel E, Kaunitz JD
Gut 2008 Dec;57(12):1654-64
Gut 2008 Dec;57(12):1654-64
Carbonic anhydrases and mucosal vanilloid receptors help mediate the hyperemic response to luminal CO2 in rat duodenum.
Akiba Y, Ghayouri S, Takeuchi T, Mizumori M, Guth PH, Engel E, Swenson ER, Kaunitz JD
Gastroenterology 2006 Jul;131(1):142-52
Gastroenterology 2006 Jul;131(1):142-52
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Carbonic Anhydrase IX in Mouse Colon. Carbonic Anhydrase IX was detected in perfusion fixed frozen sections of mouse colon using Goat Anti-Mouse Carbonic Anhydrase IX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2344) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the brush border of epithelial cells in villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
