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Western blot
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- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- NBP1-51691 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-51691, RRID:AB_11011250
- Product name
- Mouse Monoclonal Carbonic Anhydrase IX/CA9 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Carbonic anhydrase 9 confers resistance to ferroptosis/apoptosis in malignant mesothelioma under hypoxia.
Hypoxia activates cadherin-22 synthesis via eIF4E2 to drive cancer cell migration, invasion and adhesion.
Lansoprazole and carbonic anhydrase IX inhibitors sinergize against human melanoma cells.
Regional ischemic immune myopathy: a paraneoplastic dermatomyopathy.
BMP2 sensitizes glioblastoma stem-like cells to Temozolomide by affecting HIF-1α stability and MGMT expression.
Li Z, Jiang L, Chew SH, Hirayama T, Sekido Y, Toyokuni S
Redox biology 2019 Sep;26:101297
Redox biology 2019 Sep;26:101297
Hypoxia activates cadherin-22 synthesis via eIF4E2 to drive cancer cell migration, invasion and adhesion.
Kelly NJ, Varga JFA, Specker EJ, Romeo CM, Coomber BL, Uniacke J
Oncogene 2018 Feb 1;37(5):651-662
Oncogene 2018 Feb 1;37(5):651-662
Lansoprazole and carbonic anhydrase IX inhibitors sinergize against human melanoma cells.
Federici C, Lugini L, Marino ML, Carta F, Iessi E, Azzarito T, Supuran CT, Fais S
Journal of enzyme inhibition and medicinal chemistry 2016;31(sup1):119-125
Journal of enzyme inhibition and medicinal chemistry 2016;31(sup1):119-125
Regional ischemic immune myopathy: a paraneoplastic dermatomyopathy.
Cai C, Alshehri A, Choksi R, Pestronk A
Journal of neuropathology and experimental neurology 2014 Dec;73(12):1126-33
Journal of neuropathology and experimental neurology 2014 Dec;73(12):1126-33
BMP2 sensitizes glioblastoma stem-like cells to Temozolomide by affecting HIF-1α stability and MGMT expression.
Persano L, Pistollato F, Rampazzo E, Della Puppa A, Abbadi S, Frasson C, Volpin F, Indraccolo S, Scienza R, Basso G
Cell death & disease 2012 Oct 18;3:e412
Cell death & disease 2012 Oct 18;3:e412
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - Carbonic Anhydrase IX mouse antibody against Hela (1) and A549 (2) cell lysates. Bands were detected at a molecular weight of approximately 50 kDa in both cell lines.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - Paraffin-embedded lung tissues (left) and colonic tissues (right) using Carbonic Anhydrase IX mouse antibody with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - Analysis of NTERA-2 cells using Carbonic Anhydrase IX mouse mAb (green) and negative control (purple).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Carbonic Anhydrase IX/CA9 Antibody (2D3) [NBP1-51691] - A surface stain was performed on A431 cells with Carbonic Anhydrase IX/CA9 NBP1-51691 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.
