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- Antigen structure
- References [10]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- GTX70020 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX70020, RRID:AB_11173476
- Product name
- Carbonic Anhydrase IX antibody [GT12]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references Surface-Enhanced Raman Scattering Nanoparticles for Multiplexed Imaging of Bladder Cancer Tissue Permeability and Molecular Phenotype.
Inflammatory interferon activates HIF-1α-mediated epithelial-to-mesenchymal transition via PI3K/AKT/mTOR pathway.
Targeting the Mevalonate Pathway Suppresses VHL-Deficient CC-RCC through an HIF-Dependent Mechanism.
Activation of carbonic anhydrase IX by alternatively spliced tissue factor under late-stage tumor conditions.
Expression of hypoxic marker carbonic anhydrase IX predicts poor prognosis in resectable hepatocellular carcinoma.
Bortezomib attenuates HIF-1- but not HIF-2-mediated transcriptional activation.
Conditional inactivation of the mouse von Hippel-Lindau tumor suppressor gene results in wide-spread hyperplastic, inflammatory and fibrotic lesions in the kidney.
Intermediary metabolite precursor dimethyl-2-ketoglutarate stabilizes hypoxia-inducible factor-1α by inhibiting prolyl-4-hydroxylase PHD2.
E7080 (lenvatinib), a multi-targeted tyrosine kinase inhibitor, demonstrates antitumor activities against colorectal cancer xenografts.
Loss of corepressor PER2 under hypoxia up-regulates OCT1-mediated EMT gene expression and enhances tumor malignancy.
Davis RM, Kiss B, Trivedi DR, Metzner TJ, Liao JC, Gambhir SS
ACS nano 2018 Oct 23;12(10):9669-9679
ACS nano 2018 Oct 23;12(10):9669-9679
Inflammatory interferon activates HIF-1α-mediated epithelial-to-mesenchymal transition via PI3K/AKT/mTOR pathway.
Yeh YH, Hsiao HF, Yeh YC, Chen TW, Li TK
Journal of experimental & clinical cancer research : CR 2018 Mar 27;37(1):70
Journal of experimental & clinical cancer research : CR 2018 Mar 27;37(1):70
Targeting the Mevalonate Pathway Suppresses VHL-Deficient CC-RCC through an HIF-Dependent Mechanism.
Thompson JM, Alvarez A, Singha MK, Pavesic MW, Nguyen QH, Nelson LJ, Fruman DA, Razorenova OV
Molecular cancer therapeutics 2018 Aug;17(8):1781-1792
Molecular cancer therapeutics 2018 Aug;17(8):1781-1792
Activation of carbonic anhydrase IX by alternatively spliced tissue factor under late-stage tumor conditions.
Ramchandani D, Unruh D, Lewis CS, Bogdanov VY, Weber GF
Laboratory investigation; a journal of technical methods and pathology 2016 Dec;96(12):1234-1245
Laboratory investigation; a journal of technical methods and pathology 2016 Dec;96(12):1234-1245
Expression of hypoxic marker carbonic anhydrase IX predicts poor prognosis in resectable hepatocellular carcinoma.
Huang WJ, Jeng YM, Lai HS, Fong IU, Sheu FY, Lai PL, Yuan RH
PloS one 2015;10(3):e0119181
PloS one 2015;10(3):e0119181
Bortezomib attenuates HIF-1- but not HIF-2-mediated transcriptional activation.
Abd-Aziz N, Stanbridge EJ, Shafee N
Oncology letters 2015 Oct;10(4):2192-2196
Oncology letters 2015 Oct;10(4):2192-2196
Conditional inactivation of the mouse von Hippel-Lindau tumor suppressor gene results in wide-spread hyperplastic, inflammatory and fibrotic lesions in the kidney.
Pritchett TL, Bader HL, Henderson J, Hsu T
Oncogene 2015 May 14;34(20):2631-9
Oncogene 2015 May 14;34(20):2631-9
Intermediary metabolite precursor dimethyl-2-ketoglutarate stabilizes hypoxia-inducible factor-1α by inhibiting prolyl-4-hydroxylase PHD2.
Hou P, Kuo CY, Cheng CT, Liou JP, Ann DK, Chen Q
PloS one 2014;9(11):e113865
PloS one 2014;9(11):e113865
E7080 (lenvatinib), a multi-targeted tyrosine kinase inhibitor, demonstrates antitumor activities against colorectal cancer xenografts.
Wiegering A, Korb D, Thalheimer A, Kämmerer U, Allmanritter J, Matthes N, Linnebacher M, Schlegel N, Klein I, Ergün S, Germer CT, Otto C
Neoplasia (New York, N.Y.) 2014 Nov;16(11):972-81
Neoplasia (New York, N.Y.) 2014 Nov;16(11):972-81
Loss of corepressor PER2 under hypoxia up-regulates OCT1-mediated EMT gene expression and enhances tumor malignancy.
Hwang-Verslues WW, Chang PH, Jeng YM, Kuo WH, Chiang PH, Chang YC, Hsieh TH, Su FY, Lin LC, Abbondante S, Yang CY, Hsu HM, Yu JC, Chang KJ, Shew JY, Lee EY, Lee WH
Proceedings of the National Academy of Sciences of the United States of America 2013 Jul 23;110(30):12331-6
Proceedings of the National Academy of Sciences of the United States of America 2013 Jul 23;110(30):12331-6
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g HeLa whole cell lysate)A: 24 hr UntreatedB: 24 hr treatment with 100?M CoCl2C: 24 hr treatment with 200?M CoCl2D: 48 hr UntreatedE: 48 hr treatment with 100?M CoCl2F: 48 hr treatment with 200?M CoCl2Anti-CAIX antibody [GT12] (GTX70020) was used at a dilution of 1:1000The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431 cells treated with 200£gM CoCl2 for 48hr using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000. The hypoxic regions of the tumor show positive CAIX staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded renal cell carcinoma (clear cell type) using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded cervical CA tissue sections using anti-CAIX antibody [GT12] (GTX70020) at a dilution of 1:1000. The hypoxic regions of the tumor show positive CAIX staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometry on HeLa cells (1x10^6) stained with anti-CAIX antibody [GT12] (GTX70020) at a 1:1000 dilution. HeLa cells were untreated (green) or treated with 200£gM CoCl2 (pink) for 48 hr.
