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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- 500-P252-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CTGF Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: GKKCIRTPKI SKPIKFELSG CTSMKTYRAK FCGVCTDGRC CTPHRTTTLP VEFKCPDGEV MKKNMMFIKT CACHYNCPGD NDIFESLYYR KMYGDMA.
- Concentration
- 0.1-1.0 mg/mL
Submitted references Methionine Enkephalin Suppresses Osteocyte Apoptosis Induced by Compressive Force through Regulation of Nuclear Translocation of NFATc1.
Downregulation of angiotensin type 1 receptor and nuclear factor-κB by sirtuin 1 contributes to renoprotection in unilateral ureteral obstruction.
P38 pathway as a key downstream signal of connective tissue growth factor to regulate metastatic potential in non-small-cell lung cancer.
CCN2 inhibits lung cancer metastasis through promoting DAPK-dependent anoikis and inducing EGFR degradation.
Sogi C, Takeshita N, Jiang W, Kim S, Maeda T, Yoshida M, Oyanagi T, Ito A, Kimura S, Seki D, Takano I, Sakai Y, Fujiwara I, Kure S, Takano-Yamamoto T
JBMR plus 2020 Jul;4(7):e10369
JBMR plus 2020 Jul;4(7):e10369
Downregulation of angiotensin type 1 receptor and nuclear factor-κB by sirtuin 1 contributes to renoprotection in unilateral ureteral obstruction.
Yang SY, Lin SL, Chen YM, Wu VC, Yang WS, Wu KD
Scientific reports 2016 Sep 23;6:33705
Scientific reports 2016 Sep 23;6:33705
P38 pathway as a key downstream signal of connective tissue growth factor to regulate metastatic potential in non-small-cell lung cancer.
Kato S, Yokoyama S, Hayakawa Y, Li L, Iwakami Y, Sakurai H, Saiki I
Cancer science 2016 Oct;107(10):1416-1421
Cancer science 2016 Oct;107(10):1416-1421
CCN2 inhibits lung cancer metastasis through promoting DAPK-dependent anoikis and inducing EGFR degradation.
Chang CC, Yang MH, Lin BR, Chen ST, Pan SH, Hsiao M, Lai TC, Lin SK, Jeng YM, Chu CY, Chen RH, Yang PC, Chin YE, Kuo ML
Cell death and differentiation 2013 Mar;20(3):443-55
Cell death and differentiation 2013 Mar;20(3):443-55
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human CTGF by Western Blot analysis CTGF Polyclonal Antibody (Product # 500-P252-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human CTGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human CTGF by Western Blot analysis CTGF Polyclonal Antibody (Product # 500-P252-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human CTGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human CTGF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CTGF Polyclonal Antibody (Product # 500-P252-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CTGF Polyclonal Antibody, Biotin (Product # 500-P252BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human CTGF.
