AF660

antibody from R&D Systems

Targeting: CCN2 CTGF, IGFBP8

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF660

Provider

R&D Systems

Product name

Human CTGF/CCN2 C-Terminus Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human CTGF/CCN2 C-Terminus in direct ELISAs. In direct ELISAs, approximately 15% cross-reactivity with recombinant rat CTGF is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P29279


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.

Riordan DP, Varma S, West RB, Brown PO
PloS one 2015;10(7):e0128975

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SPARC mediates early extracellular matrix remodeling following myocardial infarction.

McCurdy SM, Dai Q, Zhang J, Zamilpa R, Ramirez TA, Dayah T, Nguyen N, Jin YF, Bradshaw AD, Lindsey ML
American journal of physiology. Heart and circulatory physiology 2011 Aug;301(2):H497-505

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Connective tissue growth factor and its correlation to other growth factors in experimental granulation tissue.

Inkinen K, Wolff H, Lindroos P, Ahonen J
Connective tissue research 2003;44(1):19-29

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

CTGF/CCN2 in Human Pancreas. CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human pancreas array using Goat Anti-Human CTGF/CCN2 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF660) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

CTGF/CCN2 in Human Kidney. CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human kidney array using Goat Anti-Human CTGF/CCN2 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF660) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.