Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- AP32807PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti MAPK6 / ERK3
- Antibody type
- Polyclonal
- Antigen
- Peptide with sequence from the internal region (near C-Terminus) of the protein sequence according to NP_002739.1.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Porcine
- Host
- Goat
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- HEK293 lysate (10 µg protein in RIPA buffer) overexpressing GFP-fused Mouse ERK3 probed with MAPK6 / ERK3 Antibody Cat.-No AP32807PU-N (0.5 µg/ml) in left panel and with anti-GFP in right panel. GFP-only exression in the first lane. Primary incubations were for 2 hours. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- MAPK6 / ERK3 Antibody Cat.-No AP32807PU-N (1.5 µg) Immunoprecipitation from lysates of MK5/ERK3 double knockout MEFs, with (third lane) and without (fourth lane) rescued MK5/ERK3 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with Rabbit anti-Erk3 (and co-precipitation was measured using Mouse anti-MK5/PRAK in the lower panel).